Fast Green FCF

25 g Fast Green are dissolved in 30 ml ethanol and 10 ml glacial acetic and filled up to 100 ml with deionized water B destaining solution 10% acetic acid (v/v), 30% methanol or denaturated ethanol (v/v) in deionized water 

Staining with Fast Green is partial suitable for quantitative densitometry at 625 nm. 

CA Index Name Benzenemethanaminium, A-ethyl-A-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](4-hydroxy-2-sulfophenyl)methylene]-2,5-cyclohexadien-l-ylidene]-3-sulfo-, inner salt, sodium salt (1 2) 

Physical Form Dark green or red-brown powder 

Solubility Soluble in water, ethanol insoluble in xylene 

Staining Applications Brain cytoplasm cjdoskele-ton nuclei nucleoli aluminum cells microorganisms stain for endoscopy beverages candies drinks fish sweeteners capsules ta- 

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Azo dyes Allura Red AC, Amaranth, Azorubine, Brilliant Black BN, Brown FK, Brown HT, Lithol Rubine BK, Ponceau 4R, Red 2G, Sunset Yellow, Tartrazine Triarylmethane (triphenylmethane) dyes Brilliant Blue FCF, Fast green FCF, Green S, Patent Blue V... 

Fast Green FCF (FD C Green No. 3, Cl Food Green 3) is a triarylmethane dye related to Brilliant Blue, the disodinm 3-[N-ethyl-N-[4-[[4-[N-ethyl-N-(3-snl-fonatobenzyl)amino] -phenyl] (4-hydroxy-2-snlfonatophenyl)methylene] -2,5-cyclo-hexadien-l-ylidene]ammonio-methyl]-benzenesnlfonate. Fast green is a red to brown-violet powder or crystals, solnble in water, sparingly solnble in ethanol, with a maximnm absorption in water at 625 nm. It is not permitted as food colorant in the EU. -"... 

Sastry et al. [42] reported the use of an extractive spectrophotometric method for the determination of primaquine and other antimalarial agents using Fast Green FCF (C.I. Food Green 3) or Orange II (C.I. Acid Orange 7). Sample solution... 

CZE using cyclodextrins (CD) as buffer additives has also been employed for the determination of synthetic dyes in various food products. The synthetic dyes New coccine, Erythrosine, Allura red AC, Tartrazine, Sunset yellow FCF, Brilliant blue FCF, Indigo carmine and Fast green FCF were included in the experiments. Measurements were carried out in a used-silica capillary (47 cm length, 40 cm to the detector, 50 pm i.d.). Capillary temperature was 25°C and separation voltage was 20 kV. Pressure injection was performed... 

Fast green FCF for staining polypeptides after transfer to solid support. 

Fast green stain 0.1% (w/v) Fast green FCF in 20% (v/v) methanol-5% (v/v) acetic acid. This stain is reusable. Prepare 50-100 mL/blot. 

Fast green FCF, FD C green 3 143 42053 TAM Disodium V-ethyl- V-[4[[4-[ethyl[3-sulfophenyl)methyl]-amino]phenyl](4-OH-2-sulfophenyl)methylene]-2,5-cyclo-hexadienylidene]3-sulfobenzenemethanaminium hydroxide C37H34N2Na20 oS3 808.84 628... 

Fast green FCF Excellent 20 20 Fair Poor Good Good Good... 

The dye l-Anilino 8-Naphthalene Sulfonic acid (ANS) has high specificity for protein. It fluoresces only when bound to protein [30]. In smears and handsections (i.e. unembedded materials) we have never observed it to effect emulsion stability in the manner more traditional protein dyes such as Coomassie Brilliant Blue or Fast Green often do. This relative pH independence probably is due to the mode of action of this dye. It becomes fluorescent in hydrophobic pockets on protein molecules [30] in contrast to the ionic bonding necessary for Fast Green FCF and Coommassie Blue [22]. We have not observed a strong cross-reaction with lipids, either, although a fluorescence of different spectral characteristics sometimes is seen. 

For sections of embedded material, we generally do not use ANS to localize protein. Instead we use Coomassie Brilliant Blue or Fast green FCF which are used as diachromes. Either dye is used at 0.1%W/V in 7% acetic acid [22], Slides having a puddle of stain over the sections are gently warmed for up to 5 minutes, rinsed with distilled water and allowed to dry before mounting in immersion oil for observation. Figure 5 shows an example of cream cheese treated in this manner to reveal protein. 

Fast Green FCF and Quinoline Yellow are colours permitted to be used in foods and cosmetics and have the structures shown here. Quinoline Yellow is a mixture of isomeric sulfonic acids in the two rings shown. 

Revision Entire monograph revised to reflect U.S. FDA regulations regarding certified FD C color additives. The noncertified color additive, commonly referred to as Fast Green FCF, is not permitted for use in food in the United States. 

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