Fast chromatography system

What is a fast chromatography system and do I need it for my laboratory ... 

Fast chromatography involves the use of narrow-bore columns (typically 0.1-mm i.d.) that will require higher inlet pressures compared with the conventional wide-bore capillary columns. These columns require detectors and computing systems capable of fast data acquisition. The main disadvantage is a much-reduced sample loading capacity. Advances in GC column technology, along with many of the GC-related techniques discussed below, were recently reviewed by Eiceman et... 

Principles and Characteristics As mentioned already (Section 3.5.2) solid-phase microextraction involves the use of a micro-fibre which is exposed to the analyte(s) for a prespecified time. GC-MS is an ideal detector after SPME extraction/injection for both qualitative and quantitative analysis. For SPME-GC analysis, the fibre is forced into the chromatography capillary injector, where the entire extraction is desorbed. A high linear flow-rate of the carrier gas along the fibre is essential to ensure complete desorption of the analytes. Because no solvent is injected, and the analytes are rapidly desorbed on to the column, minimum detection limits are improved and resolution is maintained. Online coupling of conventional fibre-based SPME coupled with GC is now becoming routine. Automated SPME takes the sample directly from bottle to gas chromatograph. Split/splitless, on-column and PTV injection are compatible with SPME. SPME can also be used very effectively for sample introduction to fast GC systems, provided that a dedicated injector is used for this purpose [69,70],... 

In 1995, when HPLC/MS/MS was becoming the premier tool for PK assays, chromatographic sample cycle times were typically 10 to 12 min. At 10 min per sample, 16 hr were required to process 96 samples. By 2000, scientists used shorter HPLC columns and per-sample cycle times decreased to 5 to 6 min. At 5 min per sample, it takes about 8 hr to assay one 96-well plate of samples. As a result, parallel HPLC became popular Korfmacher et al.154 described a two-column system and an MS vendor produced a triple quadrupole system designed to work with four HPLC columns.16155-158 Advances in fast chromatography continued and by 2005, sample cycle times of 1 to 2 min became common.21 87 159-161 At 2 min per sample, 3 hr are required to assay one 96-well plate of samples. 

Fast-protein or high-performance liquid chromatography systems using columns such as Mono Q, Poros HQ or TSK DEAE 5PW are suitable for the purification of immunoglobulins following this protocol. 

Automated fast flow liquid chromatography system, Akta purification system, GE Healthcare Lifesciences. 

Rates of consumption of reactants and formation of products were monitored continuously with a quadrupole mass spectrometer (Extranuclear) through a capillary sampling tube located at the outlet of the reactor, and periodically by a fast gas chromatography system (MTI M200). This GC system was capable of determining the entire product distribution through C4 hydrocarbons every 60 s. Oxygen 02 (99.997%) was obtained from Airco, Ar (99.999%) from Airco, and 20% 02 (97%) in Ar (99.998%) from ICON. 

The sample from heat treatment (28 mg of protein in 2 ml) is loaded onto a HiLoad Superdex 75 colunm (Hiannacia, 2.6 x 60 cm) connected to an FPLC (fast protein liquid chromatography) system (Pharmacia) eluted with buffer B (20 mM Tris buffer, pH 8.4,2 mMEDTA, 1 Af NaCl) at a flow rate of 2 ml/min. The active protein (18 mg) is stored at —20°. This protein is generally >90% homogeneous, as judged by (12%, w/v) SDS-PAGE. 

Turanek J, Zaluska D, Neca J. Link of a fast protein liquid chromatography system with a stirred thermostated cell for sterile preparation of liposomes by the proliposome-Uposome method appUcation to encapsulation of antibiotics, synthetic peptide immu-nomodulators, and a photosensitizer. Anal Biochem 1997 249 131-9. 

A new chromatography system, fast protein liquid chromatography... 

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