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Shuffling family

Chang CC, Chen TT, Cox BW, Dawes GN, Stemmer WP, Punnonen J, Patten PA (1999) Evolution of a cytokine using DNA family shuffling. Nat Biotechnol 17 793-797 Chen J, Baig E, Eish EN (2004) Diversity and relatedness among the type I interferons. J Interferon Cytokine Res 24 687-698... [Pg.232]

Coco, W.M. (2003) RACHITT gene family shuffling by random chimeragenesis on transient templates. Methods in Molecular Biology (Clifton, NJ), 231, 111-127. [Pg.76]

Abecassis, V., Pompon, D. and Truan, G. (2000) High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2. Nucleic Acids Research, 28, E88. [Pg.76]

Coco, W. M., RACHITT Gene family shuffling by Random Chimeragenesis on Transient Templates. Methods. Mol.Biol., 2003. 231 pp. 111-127. [Pg.216]

Family shuffling creates diverse, highly functional libraries closely related genes are required requires separation of small DNA fragments and libraries contain large percentage of unshuffled clones Stemmer, 1994... [Pg.317]

Staggered Extension Process (StEP) comparable diversity to family shuffling but with no fragment purification required same problems as family shuffling Zhao, 1998... [Pg.317]

Combinatorial Library Enhanced by Recombination in Yeast (CLERY) similar diversity to family shuffling limited to protein screening in yeast Abecassis, 2000... [Pg.317]

Single-stranded DNA shuffling higher proportion of shuffled clones than family shuffling Kikuchi, 2000... [Pg.317]

Combination of THIO-ITCHY and family shuffling (SCRATCHY) more diverse family created than either method alone useful for generating shuffling candidates where higher sequence homology is required than available genes Lutz, 2001... [Pg.318]

M. Kikuchi, K. Ohnishi, and S. Harayama, An effective family shuffling method using single-stranded DNA, Gene 2000, 243, 133-137. [Pg.336]

Kikuchi, M., Ohnishi, K., and Harayama, S. (1999). Novel family shuffling methods for the in vitro evolution of enzymes. Gene, 236, 159-167. [Pg.72]

Fig. 3. (a) Comparison of single sequence shuffling and family shuffling of cephalospo-... [Pg.276]

Christians, F. C., Scapozza, L., Crameri, A., Folkers, G., and Stemmer, W. P. C. (1999). Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling. Nature Biotechnol., 17. [Pg.287]

Family shuffling uses genes that have evolved in different microbial species from a common ancestral protein, therefore possessing sequences with a high degree of identity. This is a requirement for the process > 50% sequence identity is needed. Proteolytic enzymes like subtilisin have been shuffled, using a large family of 26... [Pg.157]

Figure 8.4. Comparison of DNA shuffling (a) and family shuffling (b). White and black dots represent advantageous and deleterious mutations, respectively. Figure 8.4. Comparison of DNA shuffling (a) and family shuffling (b). White and black dots represent advantageous and deleterious mutations, respectively.
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See also in sourсe #XX -- [ Pg.525 ]



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