Staggered extension process

ZHAO H, GIVER L, SHAO z, AFFHOLTER J A and ARNOLD F H (1998) Molecular evolutiou by staggered extension process (StEP) in vitro recombination . Nature Biotech, 18, 258-61. 

Staggered Extension Process (StEP) comparable diversity to family shuffling but with no fragment purification required same problems as family shuffling Zhao, 1998... 

Abbreviations ADCA, adipyl-cephalosporic acid epPCR, error-prone PCR IGPS, indole-3-glycerol phosphate synthase MO, monooxygenase MS, mutator strain PRAI, phosphoribosylanthranilate iso-merase ProFARI, N -[5-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxylamide ribonucleotide isomerase SDM, site-directed mutagenesis, StEP, staggered extension process... 

Figure 4-5. In vitro recombination by the staggered extension process (StEP) 11S1. Only one primer and single strands from two parents are shown. Fast annealing and extension cycles during PCR of the parent genes cause template switching of the extending strand. After full-sized recombined genes are synthesized, parent genes are removed by treatment with Dpnl.

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