DNA family shuffling

Chang CC, Chen TT, Cox BW, Dawes GN, Stemmer WP, Punnonen J, Patten PA (1999) Evolution of a cytokine using DNA family shuffling. Nat Biotechnol 17 793-797 Chen J, Baig E, Eish EN (2004) Diversity and relatedness among the type I interferons. J Interferon Cytokine Res 24 687-698... [Pg.232]

Christians, F. C., Scapozza, L., Crameri, A., Folkers, G., and Stemmer, W. P. C. (1999). Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling. Nature Biotechnol., 17. [Pg.287]

Subtilisins Various properties Improved activity stability DNA family" shuffling + screening B. subtilis [127]... [Pg.126]

Thymidine kinase Substrate specificity 7-44 fold improved specificity DNA family shuffling + screening E. coli (129]... [Pg.126]

Phytoene desaturase and lycopene cyclase New carotenoid pathway (substrate and reaction specificity) Production of torulene in E.coli DNA family shuffling+ screening E. coli [209]... [Pg.126]

Chia-Chun J. Chang, Teddy T. Chen, Brett W. Cox, Glenn N. Dawes, Willem P. C. Stemmer, Juha Punnonen and Phillip A. Patten, Evolution of a cytokine using DNA family shuffling. Nature Biotechnology, 17 (2000), 793-797. [Pg.278]

TheGillamgroupconstructedachimericlibraryofCYPlAlandCYPlA2employing restriction enzyme-mediated DNA family shuffling. They observed different activity profiles toward various luciferin derivatives among active clones, including improved specific activity, novel activities, and broadening of substrate range [147,148]. [Pg.436]

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