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Recombination family shuffling

Abecassis, V., Pompon, D. and Truan, G. (2000) High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2. Nucleic Acids Research, 28, E88. [Pg.76]

Combinatorial Library Enhanced by Recombination in Yeast (CLERY) similar diversity to family shuffling limited to protein screening in yeast Abecassis, 2000... [Pg.317]

Fig. 31.5. Gene shuffling using a single gene (1) or a family of genes (2) (A) errorprone PCR generating diversity (B) DNAse treatment (C) homologous recombination of fragments and generation of diversity. Fig. 31.5. Gene shuffling using a single gene (1) or a family of genes (2) (A) errorprone PCR generating diversity (B) DNAse treatment (C) homologous recombination of fragments and generation of diversity.
Fig. 9.5. Principle of family DNA shuffling, demonstrated on the identity network of three sequences. The thickness of the arrows corresponds to the pairwise sequence identities. The similarity of sequence A with C is low, disqualifying them from regular DNA shuffling. Addition of B acts as a bridging intermediate, having sufficient identity with either A or C to accommodate successful recombination of fragments from all three parents. Fig. 9.5. Principle of family DNA shuffling, demonstrated on the identity network of three sequences. The thickness of the arrows corresponds to the pairwise sequence identities. The similarity of sequence A with C is low, disqualifying them from regular DNA shuffling. Addition of B acts as a bridging intermediate, having sufficient identity with either A or C to accommodate successful recombination of fragments from all three parents.
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See also in sourсe #XX -- [ Pg.100 ]



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