Extracellular matrix labeling

Kim et al. (67) P. placenta Polyclonal antiserum was produced to P. placenta extracellular metabolites red spruce and birch were degraded by P. placenta using the soil-block procedure degraded wood-block samples were prepared for TEM and the immunoelectron localization of wood-degrading enzymes Extracellular membrane structures (matrix) were observed surrounding hyphae, which degraded spruce and birch wood the matrix labeled positively with antisera produced to P. placenta extracellular metabolites... 

In addition to their important roles as stored fuels (starch, glycogen, dextran) and as structural materials (cellulose, chitin, peptidoglycans), polysaccharides and oligosaccharides are information carriers they serve as destination labels for some proteins and as mediators of specific cell-cell interactions and interactions between cells and the extracellular matrix. Specific carbohydrate-containing molecules act in cell-cell recognition and... 

Hammerstedt, R.H., Keith, A.D., Snipes, W., Amann, R.P., Arruda, D., and Griel, L.C. (1978). Use of spin labels to evaluate effects of cold shock and osmolality on sperm. Biol. Reprod. 75 686-696. Hardy, D.M. and Garbers, D.L. (1995). A sperm membrane protein that binds in a species- specific manner to the egg extracellular matrix is homologous to von Willebrand factor. J. Biol. Chem. 

Antibodies recognizing both nuclear and cytosolic forms of FGF-2 have been employed in ultrastructural and cell fractionation analyses of the neuron-like adrenal chromaffin cells (Stachowiak et al., 1994 Bieger et al., 1995). Interestingly, in these studies, Nuclear (amino-terminally extended) and cytoplasmic forms were found in both nuclear and cytosolic fractions. A substantial fraction of FGF-2 was located on the outer surface of the cell membrane, consistent with the well-documented presence of FGF-2 in the extracellular matrix. Most of the cytoplasmic FGF-2 was found in endosome-like structures, while secretory granules, which had previously been reported to contain FGF-2 (Westermann et al., 1990), were devoid of label at the ultrastructural level. Accumulation of FGF in the nucleus or the cytoplasm could be triggered by activating either cAMP or protein kinase C pathways. However, the mechanism by... 

The other side of the extracellular matrix balance is the degradation of proteins by matrix metaUoproteinases (MMPs) and their control by specific tissue inhibitors. The fibrosis that results from chronic inflammation is accompanied not only by increase in collagen synthesis but also by increase in the enzymes that control degradation (46). These enzymes are involved in the pathogenesis of tumors, and inhibitors to MMPs have been labeled with F (56) and (57) for oncology imaging with PET. Unfortunately, the localization of markers developed so far in tumors appeared to be related to nonspecific binding (57). 

Wang, H.W, Le, XT, and Cheng, J.X. (2008) Label-free imaging of arterial cells and extracellular matrix using a multimodal CARS microscope. Opt Commun., 281, 1813—1822. 

The activity of extracellular superoxide dismut-ase (EC 1.15.1.1) in mouse lungs is 3- to 10-fold higher than that found in most mammals, and is 30-fold higher than that found in rat lungs (Mar-KLUND 1984). Extracellular superoxide dismutase labelling is strongest in the matrix of vessels, airways, and alveolar septa, especially in the septal tips (Fattman et al. 2000). 

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