Expression of Protein Kinase C Isozymes in Smooth Muscle

EXPRESSION OF PROTEIN KINASE C ISOZYMES IN SMOOTH MUSCLE 

A small amount of PKCe was also identified in partially purified fractions of PKC from carotid VSM (Singer, H. A., unpublished data). 

FIGURE 2 Immunoblots of VSM samples with antipeptide antibodies specific for the Ca +-independent PKC isozymes PKC8 (top) and PKC (bottom). Detection was with igQ g d auto- 

FIGURE 3 Immunoblots of swine VSM and cultured VSM cell extracts with PKCa (top) and PKCp isozyme-specific monoclonal antibodies. Lane 1 aortic extract lane 2 extract from acutely dispersed carotid VSM cells lane 3 extract from carotid VSM cells in 1° culture (Day 13) lane 4 extract from carotid VSM cells second passage (Day 11). The positions of molecular mass standards are indicated on the right. 

Evidence that PKCp is independently regulated in cells includes the observation that PKCp expression is specifically increased in rat aorta and heart in response to streptozotocin-induced diabetes (Inoguchi et al., 

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