Expression library screening

Expression library screening with sera from putatively protected hosts. 308... 

III. Purification of BUR-Binding Proteins by DNA Sequence-Specific Affinity Chromatography and Expression Library Screening... 

Expression Library Screening with a Recognition Site DNA Probe... 

Screening cDNA Expression Libraries with Antibodies... 

Young, R. A., and Davis, R. W., 1983. Efficient isolation of genes using and-body probes. Proceedings of the National Academy of Sciences U.S.A. 80 1194-1198. Using andbodies to screen protein expression libraries to isolate the structural gene for a specific protein. 

Figure 1-6-6 shows how libraries are screened to identify a desired DNA sequence. The top circle represents either a genomic library or an expression library on a growth plate. 

This pathway, which is driven by the presence of free Gfly, was modified to allow for screening of mammalian cDNA expression libraries to isolate those cDNAs that activated transcription of a pheromone-responsive promoter downstream of activated G-protein. Modifications included eliminating the pheromone receptor, replacing the endogenous yeast Ga... 

Yeast does not synthesise cycloartenol or triterpenes, and so approaches to clone plant OSCs by complementation in S. cerevisiae are not feasible because of the lack of appropriate mutants. However, LS-deficient yeast mutants accumulate high levels of 2,3-oxidosqualene, favouring the synthesis of novel cycfisation products generated by heterologous expression of OSCs. The absence of lano-sterol also facilitates analysis of the reaction products. Corey and co-workers isolated a cDNA encoding Arabidopsis thaliana CS by transforming a plant cDNA expression library into such a yeast mutant and screening protein preparations derived from pools of transformants for the ability to synthesise cycloartenol by TLC [39]. 

Harvey, B. R., Rogers, G. K., Iverson, B. I., and Georgiou, G. (2002) Anchored periplasmic expression (APEx) a new platform for library screening and affinity maturation. Conference book IBC s 13th international conference, Antibody Engineering. 

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