Expression cation exchange chromatography

Bamthouse et al. [33] demonstrated the separation of recombinant human-brain-derived neurotrophic factor (rHuBDNF) expressed in Escherichia coli in cation exchange chromatography using... 

Temperature control of the short resin column was of particular importance. At 22-24°C, results corresponded closely to values found on macrocolumns, For every 1 C rise in temperature, there is an increase of Hb Aj concentration by a 0.5% (D16) or 1% (RIO) point value. Most commercial methods supply temperature correction charts (RIO) or factors (H6). While some have found a linear relation for assay temperatures between 16 and 30°C and the increase of Hb Aj value (D16, RIO), others have reported it as only linear between 16°C and 22°C (H6). Alternatives are to use a temperature-controlled room or a Perspex water bath to take 20 to 40 minicolumns, with water at 23°C circulated from a constant-temperature water bath. The smaller the elution volume, the more critical is the influence of temperature and the necessity for strict control (S42). The results for all short-column methods should be corrected to a 23°C value and so expressed. Hammons et al. (H4) have presented a valuable evaluation of three commercial minicolumn kits. Recently, an automatic low-pressure liquid chromatographic system has been described (B18), and Diamandis et al. (Dll) have reported favorably on an automated Hb Aj. analyzer, whose separation principle is a combination of reversed-phase partition and cation-exchange chromatography. 

Purification Immunoaffmity chromatography Monoclonal antibodies expressed in plasma/albu-min-free conditions Cation-exchange chromatography Anion-exchange chromatography... 

The glucose and xylose contents were determined in the soluble TFA acid hydrolysis fraction by liquid chromatography using a cation exchanged resin (Ca" " form) column. The results are reported in Table 1. Most of the samples prepared at 300-350 C show only minor amounts of hydrolyzed material, except for samples MP-16, MP-13 and MP-14 which were prepared at low pressure or low flow rate. The percents of glucose and xylose for these samples as well as those for the samples prepared at 250 C, expressed on dry wood basis, are plotted on Figure 4. The rather well defined curve indicates that cellulose and hemicellulose are simultaneously degraded at or below 250 C. 

Levison PR, Sweater M, and Dennis JW (1999) A scale-down study into the chromatography of a peptide using the cation-exchange cellulose Express-Ion C. Journal of Chemical Technology and Biotechnology 74 204—207. 

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