Immunoaffmity chromatography

To eliminate or reduce interference and concentrate the analyte(s), the primary sample extract can further be subjected to various types of cleanup procedures including liquid-liquid partitioning, solid-phase extraction, immunoaffmity chromatography, and column-switching techniques (Table 29.17). In some instances, more than one of these procedures may be used in combination to enhance the cleanup efficiency. 

Purification Immunoaffmity chromatography Monoclonal antibodies expressed in plasma/albu-min-free conditions Cation-exchange chromatography Anion-exchange chromatography... 

Immunoassays offer a sensitive, specific, cost-effective means of screening many samples for trace residues of toxic chemicals, their metabolites, and adducts. Antibodies can be used both as detectors to quantify the amount of a chemical present and in immunoaffmity chromatography to purify and concentrate material for subsequent analysis. Applications of these assays include detection of pesticide residues, mycotoxins, biomarkers of toxicity, and industrial chemicals. 

Cheung M, Albers JJ (1984) Characterization of Upoprotein particles isolated by immunoaffmity chromatography. Particles containing A-I and A-II and particles containing A-I but not A-II. J Biol Chem 259 12201-12209... 

Battersby, J.E. Vanderlaan, M. Jones, A.J. Purification and quantitation of tumor necrosis factor receptor immunoadhe-sin using a combination of immunoaffmity and reversed-phase chromatography. J. Chromatogr. B, 1999, 728 (1), 21. 

---