Experimental methods rabbits

As a first step toward investigating this possibility, a series of experiments was conducted in which air bubbles were incubated both with rabbit plasma and with human plasma (Ward, C. A. et al. J. Appl. Plwsiol. in pre The same method of incubating the different types or plasma with air bubbles were used, but the degree of complement activation in human plasma was assessed by radioimmunoassays and that in rabbit plasma by the leukocyte aggreration test. Both experimental methods indicated similar results. 

A more wide-ranging set of experimental methods can be employed for testing in animals. Potential versus time data have been obtained over periods of 60 days [79]. Linear polarization tests have been conducted to determine corrosion rates via the Stem-Geaiy equation [50]. Potentiodynamic jx>larization tests (which are effectively impossible to conduct in humans because of safety concerns) have also been conducted in animals [81,82]. A diverse group of animal species including goats, rabbits, rats, and primates has been used for corrosion testing in vivo. 

Normal Rabbits. Six male, white rabbits (2.5 - 3.0 kg) were housed individually. Animals were fasted overnight for 16 hours (with access to water) prior to each experiment to reduce the gastrointestinal content and absorption variability. After treatment with either a control dose or experimental insulin in poly(acrylic acid) resin dose, a one week washout period was required before the next experiment. The protocol called for blood samples to be taken from an indwelling ear catheter at -1, -.5, -.25, +.5, +1, +1.5, +2, +3, +4, +5 and +6 hours. Serum glucose levels were determined by an oxidase colorimetric method using the Sigma 510 Glucose Kit. 

FIGURE 3.27 Binding of NADH with rabbit muscle lactate dehydrogenase using various methods of presenting experimental data. [Graphs reconstructed from data by Ward and Winzor, Biochem. J., 215, 685 (1983).]... 

Abstract. The significance of free radical oxidation of phospholipids in tissues of animals with experimental atherosclerosis was investigated. By using modem physico-chemical methods an elevated content of polyunsaturated fatty acids and other lipids peroxides was discovered in the blood and the aorta of rabbits with experimental atheromatosis. The human blood demonstrated a low level of protective enzymatic systems and a high content of products secondary to peroxidal oxidation of the lipids. The mechanism accounting for the action of lipids peroxides on the vascular wall resulting in the formation of atheromatous plaques is considered. 

Various experimental animal species were exposed to aerosols of CS generated by various methods from exposure from 5 to 90 min. The toxic signs observed in mice, rats, guinea pigs, rabbits, dogs, and monkeys were immediate, and included hyperactivity, followed by copious lacrimation, and salivation within... 

New experimental evidence has shown that glass capillaries filled with potassium hyaluronate solutions do not contain electrochemical activity gradients between the ends of the capillaries. Depending on the method of extraction, hyaluronic acid from rabbit skin has a molecular weight between 1.7 x 10 and 1.3 x 10. ... 

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