Enzymatic protection

Chow. C. K., and A. L. Tappel. An enzymatic protective mechanism against lipid peroxidation damage to lungs of ozone-exposed rats. Lipids 7 518-524, 1972. 

Sequential one-pot synthesis Reactions occur sequentially Variable reaction conditions (e.g., pH, T°C) No isolation of intermediate products Regioselective enzymatic protection and chemical acetylation 15... 

Kadereit, D. and Waldmann, H. (2001) Enzymatic protecting group techniquee, Chem. Rev., 101, 3367-3396. 

An intriguing observation is that MnSOD activity was retained even with the very low amounts of tissue Mn in deficient animals. These findings suggest the importance of enzymatic protection against free radical damage. It is interesting that normal levels of MnSOD were maintained in brains from Mn-deficient mice we have limited data that indicate that Mn concentration is lower than normal in brains from Mn-deficient mice. Perhaps other roles of Mn in this tissue are being compromised in order to provide adequate MnSOD activity. 

Enzymatic Protecting Group Strategies, Kadereit, D. Waldmann, H. Chem. Rev. 2001, WL 3367,... 

Enzymatic protecting-group techniques have recently been usedb in the synthesis of phosphopeptides, in which the phenylacetanoide group is removed from the respective phenylacetyl amides by means of PGA. In the course of the ensuing enzymatic transformations only the phenylacetamide-blocking group is attacked. The peptide bonds, the C-terminal ester, and the phosphates remain intact (Scheme 6). More importantly, the reaction conditions are so mild that no trace of P-elimination is observed. ... 

For enzymatic protecting group manipulations on di- and oligosaccharides in particular the use of subtilisin together with dimethylformamide as the solvent is advantageous. As has already been pointed out, the use of DMF is often critical, since... 

Figure 18-17. Selective enzymatic protection and deprotection of disaccharides.

Figure 18-22. Selective enzymatic protection of polyhydroxylated alkaloids.

The fact that the antioxidant and the enzymatic protective system complement one another in radical detoxification possibly indicates some repair properties of the antioxidants towards oxidized amino acids [6 or results from the recycling of oxidized a-tocopherol by the glutathione-ascorbate-system (7J. 

The original physiological function of PONl is involved in inactivation of toxic products produced by lipid oxidation (Draganov and La Du, 2004). In addition, PONl hydrolyzes OPCs, such as sarin, cyclosarin, tabun, and soman, thus accomplishing enzymatic protection against nerve agents in circulation (Billecke et al., 2000 Amitai et al.,... 

Reidel, A. Waldmann, H. Enzymatic Protecting Group Techniques in Bioorganic Synthesis. J. Prakt. Chem. 1993, 335, 109-127. 

Waldmann, H. Sebastian, D. Enzymatic Protecting Group Techniques. Chem. Rev. 1994,94,911-937. 

Enzymatic Protecting Group Techniques in Organic Synthesis... 

---