Examples of thermodynamically controlled reverse hydrolysis reactions

11 Examples of thermodynamically controlled reverse hydrolysis reactions 

Sealed (10 ml) reaction vessel Syringes (5 and 2 ml) and needles Gilson pipette (1000 ml) and tips Water bath and thermometer TLC equipment Rotary evaporator Freeze dryer 

Special precautions. There are no reported risks associated with the glycosidase however, many enzymes are immunogenic, so skin contact and inhalation of enzyme powders should be avoided. Safety glasses and gloves should be worn during this experiment and work should be carried out in a fiime hood. 

Notes and discussion. This synthesis is performed at high temperature and the alcohol glycosyl acceptor is used as the solvent (and therefore in huge excess) in order to increase the yield of alkyl glycoside to 60%. 

Dichloromethane for chromatography Methanol for chromatography Water for chromatography 

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