EVLS in Connection with Adsorptive Stripping Technique

4 EVLS in Connection with Adsorptive Stripping Technique 

From the definition of AdSV it follows that this method is characterized by the nature of the accumulation process, where adsorption plays an important role [28, 29]. In AdSV, the pre-concentration step is not controlled by electrolysis, but it is accomplished by anal3Ae adsorption on the working electrode surface or by reactions with chemically modihed electrodes. From the early 1960s, this technique (in connection with dc polarography and 

Adsorptive transfer stripping voltammetry (AdTSV) was introduced in 1986 as a new anal5 ical procedure based on the adsorptive pre-concentration of biomacromolecules on an electrode, the transfer of the adsorbed layer into a background electrolj e and subsequent voltammetric analysis [35]. The advantages of AdTSV were summarized as follows (i) the method utilizes differences in adsorbability of substances to their separation, (ii) due to their strong adsorption, analytes (oligonucleotides) can be separated from complex media, which are not suitable for voltammetric analysis of the conventional type, (iii) the interaction of biomacromolecules immobilized on the surface of the electrode with substances contained in the solution is possible, and (iv) all mentioned points can be affected by electrode potential [35]. 

An even higher difference was found in stirred solution when the anodic peak of guanine was measured instead of the cathodic one [33]. AdSV measuremets of nucleic acids or oligonucleotides were also performed by square wave voltammetry and ac voltammetry [36, 37]. Details about AdSV of nucleic acids were summarized in several reviews [34,38-43]. 

can considerably contribute to the electrochemical analysis of nucleic acids [7], 

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