Eversion-graft induced thrombosis

The eversion graft model for producing thrombosis in the rabbit artery was first described by Hergrueter et al. (1988) and later modified by Jang et al. (1989, 1990) and Gold et al. (1991). A 4- to 6-mm segment of the rabbit femoral or the dog left circumflex artery is excised, everted and then reimplanted into the vessel by end-to-end anastomoses. After restoration of the blood flow, a platelet-rich occlusive thrombus forms rapidly leading to complete occlusion of the vessel. 

This model mimics a deep arterial injury since the adventitial surface is a non-endothelial tissue containing tissue factor and collagen. The rabbit model described here uses a carotid graft inserted into the femoral graft to avoid vasoconstriction often occurring in the inverted femoral segments. 

Arterial blood is collected from the left carotid artery at baseline (immediately before substance administration), 10 min, 60 min and 120 min after substance administration. 

Time until occlusion (time after restoring of vessel blood flow until occlusion of the vessel indicated by a flow less than 3.0 ml/min) 

Patency (time during which perfusion of graft is measured related to an observation period of 120 min after administration of test compounds). 

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