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Eukaryotic assay systems

Examples of eukaryotic assay systems are summarised in Table 4. Among the advantages of the proposed systems is the ability to detect mitotic crossovers and non-disjunctions. [Pg.264]

It is generally, but not universally, agreed that fungal test systems cure less sensitive than bacterial test systems in almost all cases, a higher dose of the agent being tested is required to elicit a response in the fungal systems. Yeast tests cure the fastest and least expensive eukaryotic assays. [Pg.95]

In addition to the mouse lymphoma assay discussed above, several other assay systems are available for evaluating gene mutations in eukaryotic... [Pg.2686]

The two purple adenine genes, ad-3A and ad-3B, are closely linked. By using a forced heterokaryon heterozygous for the ad-3 A and the ad-3B locus, Webber and de Serres were able to show that X-ray-induced specific-locus mutations in the ad-3 region were either point mutations or chromosome deletions (Webber and de Serres, 1965). The same classes of mutations are picked up in the specific-locus system in mice (Russell, 1951,1967). To sunmiarize in de Serres purple adenine system we have a eukaryotic, microbial assay system in which the same type of mutations can be isolated as in mammals. However, because it is a microbial system, it is considerably easier to perform quantitative mutagenesis studies with this system than with mice. [Pg.279]

The principles and methodology available for the use ofprokaryotes and eukaryotes whose cell nuclei respectively are not and are enclosed by membranes) as genetic assay systems will be summarised. Selected examples include reversion and forward-mutation assay systems. A recent theoretical analysis with limited experimental data will be presented, which describes how a plate diffusion assay may be used to study the stability of mutagenic chemicals. [Pg.251]

Methyl parathion has been tested in numerous genotoxicity assays using prokaryotic and eukaryotic systems with both positive and negative results. Results of these studies are summarized in Tables 3-5 and 3-6. [Pg.82]

Studies on the genotoxicity of methoxychlor have generally yielded negative results in prokaryotic assays, mixed results in in vitro eukaryotic systems, and negative results in in vivo studies. ... [Pg.444]

Mustard gas is highly genotoxic. In vitro assays in both prokaryotic and eukaryotic systems support a mechanism of DNA alkyla-... [Pg.502]

To validate interaction assays, a suitable biological model system is helpful. cAMP-dependent protein kinase (PKA) is used as such a model system in the assays described below. PKA is the main target for the second messenger cAMP in eukaryotic cells and, since it has been implicated in various human diseases, the development of cAMP analogs modulating PKA activity has provoked increasing interest. [Pg.161]


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Assay systems

Eukaryotic systems

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