Ethanol ester biosynthesis from

Effect of Inhibition of Acetyl-CoA Formation on Ethyl Acetate Accumulation. Ethyl acetate has been shown to be formed from acetic acid and ethanol without any cofactors in S. cerevlslae suggesting an esterase mechanism of biosynthesis (V7). Other studies (18) found cell-free synthesis of ethyl acetate with ethanol and acetyl-CoA but not with acetic acid as a substrate. From the latter study it was proposed that ester formation in yeasts was primarily via alcoholysis of acyl-CoA compounds. Others have also suggested acetyl-CoA as an intermediate for ethyl acetate accumulation by S. cerevlslae (19). 

More recently we have measured the turnover rate of lAA in tomato shoots after different incubation times, as part of a study of lAA biosynthesis in that tissue. lAA was supplied to the surface of the youngest leaf, 1 cm or longer, of excised shoots from twenty, 4-week-old tomato plants, as a 10 /xl drop (2 1 1, ethanol propan-2-ol H2O) containing 3711 Bq pH]IAA (1.11 GBq//xmol). Uptake was allowed for 2 h, surface radioactivity was then removed by several washes with 50% aqueous propan-2-ol followed with water, and the plants equilibrated for 1 h. After 0,4,10 or 20 h incubation in continuous light, lAA was extracted, purified, derivatised to form the pentafluorobenzyl ester [10] and the specific activity measured by liquid scintillation counting and GC-ecd. The purity and identity of selected samples was confirmed by combined GC-MS. 

---