Escherichia coli transcription

Kaldalu, N., Mei, R., Lewis, K. Killing by ampicillin and ofloxacin induces overlapping changes in Escherichia coli transcription profile. Antimicrob Agents Chemother 48 (2004) 890-896. 

B.L. Stitt. 2001. Escherichia coli transcription termination factor Rho hinds and hydrolyzes ATP using a single class of three sites Bio-chemistry 40 2276-2281. (PubMed)... 

Mukhopadhyay P, Zheng M, Bedzyk LA, LaRossa RA, Storz G. 2004. Prominent roles of the NorR and Fur regulators in the Escherichia coli transcriptional response to reactive nitrogen species. Proc Natl Acad Sci USA 101(3) 745-750. 

Hitchens, T.K., Zhan, Y, Richardson, L.V., Richardson, J.P., and Rule, G.S. 2006. Sequence-specific interactions in the RNA-binding domain of Escherichia coli transcription termination factor Rho. /. Biol. C/zem., 281, 33697-33703. 

More than 30 years ago Jacob and Monod introduced the Escherichia coli lac operon as a model for gene regulation. The lac repressor molecule functions as a switch, regulated by inducer molecules, which controls the synthesis of enzymes necessary for E. coli to use lactose as an energy source. In the absence of lactose the repressor binds tightly to the operator DNA preventing the synthesis of these enzymes. Conversely when lactose is present, the repressor dissociates from the operator, allowing transcription of the operon. 

The chromosomes of Escherichia coli and other bacteria are single, double-stranded DNA molecules with a total length of more than 1,000 pm. Relaxed DNA exists as a helical molecule, with one full turn of the helix occurring approximately every 10.4 base pairs. This molecule must undergo several folding and compaction steps to fit into an E. coli cell which is only 1-3 pm long. Despite this enormous compaction, bacterial DNA must be accessible for the bacterial enzymes that catalize DNA replication and transcription... 

Lee, L.J., Barrett, J.A., and Poole, R.K., Genome-wide transcriptional response of chemostat-cultured Escherichia coli to zinc, J Bacteriol, 187 (3), 1124-1134, 2005. 

Worden, C., Kovac, W., Dorn, L., and Sandrin, T., Environmental pH affects transcriptional responses to cadmium toxicity in Escherichia coli K-12 (MG1655), FEMS Microbiol Lett, 293 (1), 58-64, 2009. 

Brocklehurst, K.R. and Morby, A.P., Metal-ion tolerance in Escherichia coli Analysis of transcriptional profiles by gene-array technology, Microbiology, 146 (9), 2277-2282, 2000. 

Sheridan, G. E. C. Masters, C. I. Shallcross, J. A. Mackey, B. M. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl. Environ. Microbiol. 1998, 64,1313-1318. 

Harada Y, Ohara O, Takatsuki A, Itoh H, Shimamoto N, Kinosita K (2001) Direct observation of DNA rotation during transcription by Escherichia coli RNA polymerase. Nature 409 113-115... 

Self, W. T., Grunden, A. M., Hasona, A., and Shanmugam, K. T. (1999). Transcriptional regulation of molybdoenzyme synthesis in Escherichia coli in response to molybdenum ModE-molybdate, a repressor of the modABCD (molybdate transport) operon is a secondary transcriptional activator for the hyc and nar operons. Microbiology 145, 41-55. 

Atlung, T., Knudsen, K., Heerfordt, L. and Broendsted, L. (1997) Effects of sigma(S) and the transcriptional activator AppY on induction of the Escherichia coli hya and cbdAB-appA operons in response to carbon and phosphate starvation./o r a/ of Bacteriology, 179,2141-6. 

Richard, D. J., Sawers, G., Sargent, E, McWalter, L. and Boxer, D. H. (1999) Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydro-genases 1 and 2 oi Escherichia coli. Microbiology, 145, 2903-12. 

Schwartz CJ, Giel JL, Patschkowski T, et al. 2001. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc Natl Acad Sci USA 98 14895-900. 

Williamson, P. and Felsenfeld, G. (1978) Transcription of histone-covered T7 DNA by Escherichia coli RNA polymerase. Biochemistry 17, 5695-5705. 

Gralla JD, Escherichia coli ribosomal RNA transcription Regulatory roles for ppGpp, NTPs, architectural proteins and a polymerase-binding protein. Mol Microbiol 55 973-977, 2005. 

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