Escherichia coli phospholipases

A number of allergens from both honey bee and vespid venoms have been cloned and expressed by either Escherichia coli or baculovirus-infected insect cells (table 1) phospholipase Aj [20], hyaluronidase [21], acid phosphatase [13] and Api m6 [14] from honey bee venom, as well as antigen 5 [22], phospholipase A and hyaluronidase [23] from vespid venom, and dipeptidylpeptidases from both bee and Vespula venoms [15, 16]. Their reactivity with human-specific IgE antibodies to the respective allergens has been documented [11-16, 22, 23] and their specificity is superior... 

Franken, P.A., et al. 1992. Purification and characterization of a mutant human platelet phospholipase A2 expressed in Escherichia coli. Eur J Biochem 203 89. 

Deng, T., Noel, J. P., and Tsai, M.-D. (1990). A novel expression vector for high-level synthesis and secretion of foreign proteins in Escherichia coli Overproduction of bovine pancreatic phospholipase A2. Gene 93, 229-234. 

Dudler, T., Chen, W.-Q., Wang, S., Gmachi, M Dempcy, R., Annand, R., Crameri, R., Suter, M., and Gelb, M. H. (1992). High-level expression in Escherichia coli and rapid purification of enzymatically active phospholipase A2 from honey bee venom. Biochim. Biophys. Acta 1165,201-210. 

Kelley, M. J., Crowl, R. M., and Dennis, E. A. (1992). Renaturation of cobra venom phospholipase A2 expressed from a synthetic gene in Escherichia coli. Biochim. Biophys. Acto 1118,107-115. 

Two PLAs have been purified from Escherichia coli based on their differential sensitivity to treatment with detergents [2]. A detergent-insensitive enzyme is localized in the outer membrane, whereas a detergent-sensitive enzyme is found on the cytoplasmic membrane and in soluble fractions. The outer membrane enzyme, known as outer membrane phospholipase A, has broad substrate specificity and demonstrates PLA, PLAj, lysophospholipase A, and lysophospholipase Aj activity as well as activity for hydrolyzing monoacylglycerols and diacylglycerols. The crystal structure allows a more detailed discussion of an integral membrane phospholipase [12]. 

Pedersen, P.B. (2005) Phospholipase, PPW23436—Test for mutagenic activity with strains o/Salmonella typhimurium and Escherichia coli. Unpublished report No. 20048085 from... 

Givskov, M. Olsen, L. Molin, S., Cloning and Expression in Escherichia coli of the Gene for Extracellular Phospholipase A1 from Serratia liquefaciens. J Bacterial 1988, 170, 5855-5862. 

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