Escherichia coli infections sepsis

Tinospora cordifolia has been evaluated in several animal models for immunosuppression. In a model of irreversible cholestasis, Escherichia coli sepsis was created [22]. Rats from the Tinospora cordifolia-lrtdXtd groups resisted Escherichia coli infection and mortality was only 16.7%. Blood cultures of the surviving animals were sterile. In comparison, the control rats showed a mortality of 77.8 % and the blood culture of surviving animals from these groups showed the presence of Escherichia coli. 

Characterization of Anti-Infective Biological Activity. Acute sepsis models utilizing either Escherichia coli or Staphylococcus aureus intraperitoneal challenge were developed to evaluate the anti-infective properties of PGG in mice. 

Korhonen, T. K., Valtonen, M. V., Parrkinen, J., Vaisanen-Rhen, V., Finne, J., Orskov, L, Svenson, S. B., and Makela, P. H. (1985). Serotypes, hemolysin production, and receptor recognition of Escherichia coli strains associated with neonatal sepsis and meningitis. Infect. Immun. 48,486-491. 

Bhattacharjee, A.K., Opal, S.M., Palardy, J.E., Drabick, J.J., Collins, H., Taylor, R., Cotton, A., Cross, A. Affinity-purified Escherichia coli J5 lipopolysaccharide-specific IgG protects neutropenic rats against gram negative bacterial sepsis. J Infect Dis 170 (1994) 622-629. 

Bacteria The choice of bacteria for animal models of sepsis depends on the goals of the study. We have used Escherichia coli, because it is one of the most common clinical isolates in humans with sepsis (5). The K-l serotype is associated with invasive infection in humans and rabbits. Bacteria are thawed from a frozen stock and inoculated into 50 mL Lennox-B broth, then grown overnight to stationary phase at 37°C with continuous stirring. After overnight incubation, the... 

Korhonen TK, Valtonen MV, Parkkinen J et al. (1985) Serotypes, hemolysin production, and receptor recognition of Escherichia coli strains associated with neonatal sepsis and meningitis. Infect Immun 48 486—491... 

Briefly, Tinospora cordifolia reduced mortality associated with intra-abdominal sepsis following caecal ligation. Thus, as compared to a mortality of 66 100% on the fifth day after surgery in untreated rats, 15 days of pretreatment with Tinospora cordifolia alone reduced mortality to 33% and further to 16.6% when combined with metronidazole and gentamicin [19]. This effect was reported to be associated with improved macrophage function. Furthermore, the aqueous extract of Tinospora cordifolia was found to be devoid of in vitro antimicrobial activity against Bacillus subtilis, Bacillus cereus Staphylococcus aureus Pseudomonas aeruginosa and Escherichia coli. Sera obtained from rats (n = 10 each) orally treated with the aqueous extract of Tinospora cordifolia in doses of 100 and 200 mg/kg were also found to have no antibacterial activity [19]. These data further indicated that Tinospora cordifolia was effective in infections due to its immunostimulant effects. 

Guenther K, Straube E, Pfister W, Guenther A, Huebler A. Severe sepsis after probiotic treatment with Escherichia coli NISSLE1917. Pediah Infect Dis J 2010 29(2) 188-9. 

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