Escherichia coli genotyping

Goodacre, R. Berkeley, R. C. W. Detection of small genotypic changes in Escherichia coli by pyrolysis mass spectrometry. FEMS Microbiol. Lett. 1990, 71, 133-137. 

J. S. Edwards and B. 0. Palsson, The Escherichia coli mgl655 in silico metabolic genotype Its definition, characteristics, and capabilities. Proc. Natl. Acad. Sci. USA 97(10), 5528 5533 (2000). 

Dixit, S. M., Gordon, D. M., Wu, X. Y., Chapman, T., Kailasapathy, K., and Chin, J. J. (2004). Diversity analysis of commensal porcine Escherichia coli—Associations between genotypes and habitat in the porcine gastrointestinal tract. Microbiology 150,1735-1740. 

Johnson, L. K., Brown, M. B., Carruthers, E. A., Ferguson, J. A., Dombek, P. E., and Sadowsky, M. J. (2004). Sample size, library composition, and genotypic diversity among natural populations of Escherichia coli from different animals influence accuracy of determining sources of fecal pollution. Appl. Environ. Microbiol. 70,4478M485. 

Meacham, K. J., Zhang, L., Foxman, B., Bauer, R. J. and Marrs, C. F. (2003) Evaluation of genotyping large numbers of Escherichia coli isolates by enterobacterial repetitive intergenic consensus-PCR. J. Clin. Microbiol. 41, 5224-5226. 

Bacterial Cultures. Bacterial cultures were obtained from the Kansas State University (KSU) stock culture collection and were stored using the Protected Bead storage system. The following cultures were used for the Salmonella specimen S. lille (UGA), S. montevideo (UGA), S. typhimurium (UGA), S. agona (KSU 05 from CDC outbreak isolate), and S. newport (KSU 06 CDC outbreak isolate). The following cultures were used for the Escherichia coli specimen E. coli 0157 H7 (CDC 01,03), E. coli 0157 H7 (USDA-FSIS 011-82 Rif resistant 100 ppm), E. coli 0157 H7 (ATCC 43895 HUS associated Type I II toxins Rif. Res.) and E. coli ATCC 23740 (Genotype K-12 prototrophic lambda). 

Additional support for DNA as the bearer of genetic information came from studies by Hershey and Chase (1952) in the replication of DNA-containing bacteriophages. Using virus particles isotopically labeled in either the viral DNA or in the viral protein, it was shown that the viral DNA entered the host cell (Escherichia coli), whereas the viral protein did not. Later experiments demonstrated that viral DNA alone was infectious and led to the formation of mature infectious virus particles of the appropriate genotype. The DNA contained all the information required for the synthesis of progeny phage particles. 

Frosch M, Roberts I, Gorgen I, Metzger S, Boulnois GJ, Bitter-Suermaim D (1987) Serotyping and genotyping of encapsulated Escherichia coli Kl sepsis isolates with a monoclonal IgG anti Kl antibody and Kl gene probes. Microb Pathog 2 319-326... 

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