Escherichia coli genetically engineered

Kelly, R. F. and Winkler, M. E. (1990) Folding of eukaryotic proteins produced in Escherichia coli. Genetic Engineering 12(1), pp. 1-9. 

Bernstein HC, Paulson SD, Carlson RP (2012) Synthetic Escherichia coli consortia engineered for syntrophy demonstrate enhanced biomass productivity. J Biotechnol 157 159-166 Blank LM, Kuepfer L (2010) Metabolic flux distributions genetic information, computational predictions, and experimental validation. Appl Microbiol Biotechnol 86 1243-1255 Bloom AJ, Chapin FS, Mooney HA (1985) Resource Umitation in plants - an economic analogy. Annu Rev Ecol Syst 16 363-392... 

Deng X., Li QB, Lu YH, Sun DH, Huang YL, Chen XR (2003) Bioaccumulation of nickel from aqueous solutions by genetically engineered Escherichia coli. Water Res 37 2505-2511... 

Anderson, J. P., Cappello.J., and Martin, D. C. (1994). Morphology and primary crystal-structure of a silk-like protein polymer synthesized by genetically-engineered Escherichia-coli bacteria. Biopolymers 34, 1049-1058. 

Researchers from the University of Maryland in Baltimore (Maryland), the Boyce Thompson Institute for Plant Research in Ithaca (New York), and Tulane University in New Orleans (Louisiana) have performed the hrst human trial of edible vaccine. Potatoes were genetically engineered to produce a diarrhea-causing toxin secreted by the bacterium Escherichia coli. 

The Gram-negative bacterium Escherichia coli (E. coli) is a usually harmless symbiont in the intestine of mammals. The structure and characteristics of this organism have been particularly well characterized. E. coli is also frequently used in genetic engineering (see p. 258). 

Shikim. shikimic and quinic acids (genetically engineered Escherichia coli Draths 1999). 

A. General description Intron A (interferon alfa-2b recombinant) is a recombi-nantly derived water-soluble protein with a molecular weight of 19.3 kDa. It is obtained from the bacterial fermentation of a strain of Escherichia coli bearing a genetically engineered plasmid containing an interferon alfa-2b gene from human leukocytes. 

Recorbet, G., Picard, C., Normand, P. Simonet, P. (1993). Kinetics of the persistence of chromosomal DNA from genetically engineered Escherichia coli introduced to soil. Applied and Environmental Microbiology, 59, 4289-94. 

Khan, A. A. Walia, S. K. (1992). Use of genetically engineered Escherichia coli strain to produce 1,2-dihydroxy-4 -chlorobiphenyl. Applied and Environmental Microbiology, 58, 1388-91. 

C. -H. Chou, G. N. Bennett, and K. Y. San, Effect of modified glucose uptake using genetic engineering techniques on high-level recombinant protein production in Escherichia coli dense cultures, Biotechnd. Bioeng. 1994, 44, 952—960. 

Ricin is a type II toxin. The A chain (ricin A) contains 267 amino acid residues, and the B chain (ricin B) 262 residues. Ricin A is exceptionally toxic, and it has been estimated that a single molecule is sufficient to kill an individual cell. This peptide can be prepared by genetic engineering using Escherichia coli. The potent action of this material on eukaryotic cells has been investigated in anticancer therapy. Ricin A has been coupled to monoclonal antibodies and successfully delivered specifically to the tumour cells. However, in vitro toxicity of ricin A-based immunotoxins is enhanced significantly if ricin B is also present. 

J Bartholome-De Beider, M Nguysen-Disteche, N Houba-Herin, JM Ghuysen, IN Maruyama, H Hara, Y Hirota, M Inouye. Overexpression, solubilization, and refolding of a genetically engineered derivative of the penicillin-binding protein 3 of Escherichia coli K12. Mol Microbiol 2 519-526, 1988. 

LCS Ferreira, U Schwarz, W Keck, P Charlier, O Dideberg, JM Ghuysen. Properties and crystallization of genetically engineered, water-soluble derivative of penicillin-binding protein 5 of Escherichia coli K12. Eur J Biochem 171 11-16, 1988. 

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