Eriocitrin

Different extracts of Citrus were subjected to SPE on Cig cartridges to remove polar components. The retained flavonoids (mainly flavanones) were eluted with methanol-dimethyl sulfoxide, which enhanced solubility of hesperidin, diosmin, and diosmetin. Recoveries of eriocitrin, naringin, hesperidin, and tangeretin from spiked samples of mesocarp tissue exceeded 96%. Flavones were relatively abundant in the leaves. ... 

For several phenolics from citrus, such as eriocitrin, poncirin, and sinapic acid and anthocyanins, which occur in red grapefruit varieties and blood oranges, no specific drug interactions and also no interactions with CYP3A4 and P-gp are reported. 

Dilute citrus juice (5 ml) with dimethylformamide (DMF, 10 ml) and 50 mM ammonium oxalate (10 ml), and then steam-bath for 10 min at 90°C. Cooling, adjust the volume to 50 ml with water, centrifuge (10 min at 2500 g), and then filter (Acrodisc, 5 and 0.45 /nm) the clarified juice. For standard preparation, prepare hesperidin (20 ppm) in DMF-water (2 1) solution and other flavanone glycosides (neohesperidin, eriocitrin, neoeriocitrin, naringin, and narirutin, 10 ppm) in the mobile phase. 

For lemon juice, the flavonoid composition was characterized by HPLC with photodiode detection at 287 nm (108), the HPLC condition based on a procedure proposed by Kirksey et al. (103) for the detection of fruit juice adulteration. Hesperidin and eriocitrin were the characteristic flavonoids of lemon juice. Flavonoid content by HPLC was used to study the effects of processing and pulp removal on flavonoid composition in lemon juice. Eriocitrin is also used in distinguishing lemon juice from grapefruit and orange juices, which do not contain this flavonoid. 

Citrus juice Narirutin, (neo) eriocitrin (neo) hesperidin, naringin, didymin Freeze-dried, extracted with ether or hexane, followed by EtAc, MeOH or ACN C-18 MeOH-H20-acetic acid HPLC/DAD (280 nm) 7-18 mg/L 11... 

M22. Miyake, Y., Shimoi, K., Kumazawa, S., Yamamoto, K., Kinae, N., and Osawa, T., Identification and antioxidant activity of flavonoid metabolites in plasma and urine of eriocitrin-treated rats. 

Eriocitrin (= 5,7,3, 4 - Tetrahydroxyflavanone-7-O- rutinoside) (flavanone O-glycoside) Mentha piperita (Lamiaceae), Myoporum tenuifolium (Myoporaceae), Citrus spp. (Rutaceae) ACE (28% inhibition at 300 pM) [76]... 

Eriodictyoi (= 5,7,3, 4 -Tetrahydroxyflavanone) (flavanone) Eriodictyon californicum (Hydrophyllaceae) Asteraceae, Fabaceae, Lamiaceae as 7-rhamnoside (Eriocitrin) Citrus spp. (Rubiaceae) AROM (0.6) (EST-R)... 

Eriocitrin, neoeriocitrin, narirutin, naringin, hesperidin, neohesperidin... 

Red Orange juice Eriocitrin, hesperidin, narirutin, naringin, neoeriocitrin, neohesperidin Ion spray (IS), Turbo ion spray (TIS) negative ion mode Careri et al., 1999... 

Fig. 2 Separation of 25 flavonoid standards (1—eriocitrin 2— neoeriocitrin 3—robinetin 4—narirutin 5—naringin 6— rutin 7— hesperidin 8—neohesperidin 9—isorhoifolin 10— rhoifolin 11—diosmin 12—neodiosmin 13—neoponcirin 14—quercetin 15— poncirin 16—luteolin 17—kaempferol 18—apigenin 19—isorhamnetin 20—diosmetin 21— rhamnetin 22—isosakuranetin 23— sinensetin 24—acacetin 25—tangeretin) using Cig Lichrospher 100, 250 x 4.0 mm, 5 pm, Merck gradient elution with 0.01 M phosphoric acid-methanol with flow rate of 0.6 mL/min at 40°C and detection at 285 nm. (From Ref. [8].)...

Pig. I Aiiranlii pericarpiurn (1) two characteristic red-orange zones of tlavonoid glycosides naringin/neohesperidin (bitter), rutin/eriocitrin (non-bitter) at jRf 0,4-0.5. 

Flavanon glycosides eriocitrin, naringin, hesperidiii (see Aurantii pericarpiuni)... 

The higher amount of naringin, neohesperidin and hesperidin in Aurantii pericarpium (2) is seen as a broad yellow band directly above the violet-red eriocitrin. The R, value depression is caused by other plant products. 

Since the very beginning, citrus flavonoids have awakened the interest of chemists. Thus, hesperidin was reported for the first time by Lebreton in 1928 [10], Nowadays it is widely accepted that hesperidin, neohesperidin, and eriocitrin are the major flavonoids in citrus fruits. Other flavanones that may be found, although in lower percentages, are isonaringin and isosakuranetin. Likewise, other compounds that occur as... 

Lemon Hesperidin, eriocitrin rutin, diosmin 6,8-di-C-glucosyl diosmetin, 6-C-glucosyldiosmetin ... 

Sweet orange hesperidin, narirutin, eriocitrin, isosakuranetin 7-rutinoside sinensetin, nobiletin, tangeretin, heptamethoxyflavone tetramethoxyflavone isosinensetin... 

Fig. (6). HPLC chromatogram of lemon juice phenolics (1) eriocitrin, (2) diosmetin 6,8-di-C-glucoside, (3) hesperidin, and (4) diosmetin 6-C-glucoside. HPLC conditions RP C g column (12 X 0.4 cm particle size 5 (im). Mobile phase acidified water (5% formic acid) (A) and methanol (B). Gradient 0 min- 10% B, 30 min- 40% B, 40 min- 80% B. Flow rate 1 mL/min.

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