Epitope presentation systems

Two types of expression systems based on plant RNA viruses have been developed for production of immunogenic peptides and proteins in plants epitope presentation systems (short antigenic peptides fused to the CP that are displayed on the surface of assembled viral particles) and polypeptide expression systems (these systems express the whole unfused recombinant protein that accumulates within the plant). 

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Virus infected plants Yield, timescale, mixed infections, epitope presentation systems Construct size limitations... 

FIGURE 4.2 Schematic diagram of RNA virus expression vectors, (a) TMV as an epitope presentation system, and (b) a polypeptide presentation system. Dark diamonds represent foreign antigen/peptide. 

IgG responses in mice, suggesting that this VLP composed of HBsAg is an excellent system for epitope presentation and mucosal delivery. 

We present, here, the workflows for Tag-Mass of antigens and mRNA using a photocleavable probe bearing a peptide as reporter moiety. For antibodies, preference was given to use indirect IHC with a primary-secondary antibody system. Indeed, indirect IHC is known to present better performances by decreasing steric obstruction problems and increasing detection level, since secondary antibodies will recognize consensus epitope present in the... 

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