Enzyme responsive polymers chemical properties

Besides the chemoenzymatic approaches described above, where functional groups are added to allow for conjugation with other macromolecules, enzymes demonstrate other possibilities to obtain peptide- and protein-polymer conjugates, which are normally difficult to obtain by common chemical synthetic strategies. Enzyme responsiveness can be used to modulate bioconjugate properties in order to create hybrid materials with... 

Characterisation of the enzyme-responsive material prepared through the methods outlined above is essential not only to test the enzyme responsiveness of the polymer but also to characterise the material s overall performance under the conditions in which it will be used in its ultimate application. This section will provide a brief overview of standard and specialised techniques that have been employed for this purpose and covers mechanical, chemical, physical and biological properties as well as enzyme responsiveness. While we will discuss the reason for choosing particular techniques and explain their advantages and Umitations in the context of the analysis of enzyme-responsive materials, explanations of the working principles of the techniques will not be provided and the reader is referred to other specialised textbooks on this topic instead. 

Fixation substrate functions depend largely on molecular level structure and properties such as chemical and stereospecific structure, the structure and property of supramolecular materials, and macroscopic properties like high porosity. In addition to the progress already made in both s)mthesis of functional polymers such as stimuli-responsive and molecular recognition types and development of composite manufacturing methods, if morphological characteristics can be utilized, enzyme reactions will be possible anytime they are needed. 

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