Enzyme-Linked Lectin Assay ELLA

To quantify their binding to WGA in solution, glycopeptides 16, 18, and 22 were re-synthesized as single compounds. IC50 values for inhibition of the binding of porcine stomach mucin to peroxidase-labeled WGA (Table 2.7.1) were determined by an enzyme-linked lectin assay (ELLA) as described by Zanini and Roy [21]. The IC50 values shown in Table 2.7.1 confirm that the binding behavior of 16, 18, and... 

McCoy JP, Varani J, Goldstein U (1984) Enzyme-linked lectin assay (ELLA). 2. Detection of carbohydrate groups on the surface of unfixed cells. Exp Cell Res 151(1) 96-103... 

Discussion of the effect of ligand structure on protein-carbohydrate affinity requires an evaluation of complex stability constants. A munber of biophysical techniques are appropriate for the study of protein-carbohydrate interaction many of the more enlightening strategies are the topics of separate chapters elsewhere in this volume. We describe below three techniques used extensively in glycobiology— inhibition of hemagglutination, enzyme-linked lectin assay (ELLA), and isothermal titration microcalorimetry—and we consider the types of information provided by each technique in order to facilitate appropriate interpretation of the data. 

ELLA enzyme-linked lectin inhibition assay EcorL Erythrina corallodendrum... 

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