Enzyme in the Mammalian Cell, with Particular Reference to Activity Measurements

Location of the Enzyme in the Mammalian Cell, with Particular Reference to Activity Measurements 

The effect of high tissue-concentrations must be distinguished from the action of the insoluble, endogenous inhibitor in rat-tissue homogenates, which cannot be abol- 

Effect of Varying the Concentration of a Water Homogenate of Normal Adult Mouse Liver, Buffered with Acetate, During the Incubation with Substrate (0.00126 M Phenolphthalein fl-v-Glucosiduronic Acid).33 Homogenate Fractionated Before and After Incubation at 88°, and then Assayed 

Final Concentration of Moist Liver in Assay (%) Net Activity (units/g. liver)  

Observations made in studying the behavior of /3-glucuronidase in homogenates in isotonic media33-162 closely resembled the results obtained in contemporary work on acid phosphatase by de Duve and his colleagues,163166 who later confirmed the work on /3-glucuronidase in many of its essential 

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