Enzyme Activity and Inhibition Studies

The development of efficient methods to assay molecules for catalytic enzyme activity is of considerable importance in drug discovery. In this respect, MS has 

The same MALDI-based assay approach with DHB butylamine (DHBB) was implemented to monitor the desialylation reaction of 3 -sialyUactose [128]. DHBB is a liquid ionic matrix prepared by mixing equimolar amounts of DHB and butylamine, and can be used in lieu of an organic solvent in the enzyme reaction, thereby allowing rapid monitoring of the enzyme-catalyzed reaction. 

The appHcabihty of DIOS-MS to monitor enzyme activity and inhibition studies has been reported [144, 145]. The viability of this system to monitor enzyme activ- 

Quantitative Analysis of Samples from Complex Biological Matrices 

Sample clean-up requirements stiU remain strict for MALDI analysis, with even low protein/Hpid/carbohydrate matrices such as human urine demanding a two-step liquid/Hquid extraction protocol [36]. However, reports of MALDI 

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