Enhanced Characterization and Comprehensive Analyses by Two-Dimensional Chromatography

Enhanced Characterization and Comprehensive Analyses by Two-Dimensional Chromatography 

Multidimensional chromatography tackles the root cause of limited chromatographic resolution by vastly improving the peak capacity, this being the product of the peak capacities, ft, of the individual chromatographic separations (Eq. 1)  

(1) is a simplification for the sake of clarity based on isocratic elution and orthogonal separation techniques a more general qttantification is given in Ref [1]. 

This type of separation is extremely useful for any kind of complex samples that are difficult to separate, identify comprehensively, or fully deformtjlate. This is most often the case for synthetic, natural, and biopolymers (monodisperse proteins are one well-known exception), which possess coexisting mtdtiple property 

HPLC Made to Measure A Practical Handbook for Optimization. Edited by Stavros Rromidas 

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