Endothelial microparticles

Representative Protocols of Analysis of Endothelial Microparticles Used in Clinical Studies... 

Representative Findings of Levels of Endothelial Microparticles in Different Disorders... 

Horstman LL, Jy W, Jimenez JJ, Ahn YS. Endothelial microparticles as markers of endothelial dysfunction. Front Biosci 2004 9 1118-1135. 

Combes V, Simon AC, Grau GE, Arnoux D, Camoin L, Sabatier F, Mutin M, Sanmarco M, Sampol J, Dignat-George F. In vitro generation of endothelial microparticles and possible prothrombotic activity in patients with lupus anticoagulant. J Clin Invest 1999 104 93-102. 

Jimenez JJ, Jy W, Mauro LM, Horstman LL, Ahn YS. Elevated endothelial microparticles in thrombotic thrombocytopenic purpura findings from brain and renal microvascular cell culture and patients with active disease. Brit J Haematol 2001 112 81-90. 

Brodsky SV, Malinowski K, Golightly M, Jesty J, Goligorsky MS. Plasminogen activator inhibitor-1 promotes formation of endothelial microparticles with procoagulant potential. Circulation 2002 106 2372-2378. 

Dignat-George F, Camoin-Jau L, Sabatier F, et al. Endothelial microparticles A potential contribution to the thrombotic complications of the antiphospholipid syndrome. Thromb Haemost 2004 91 667-673. 

Tramontane AF, O Leary J, Black AD, Muniyappa R, Cutaia MV, El-Sherif N. Statin decreases endothelial microparticle release from human coronary artery endothelial cells Implication for the Rho-kinase pathway. Biochem Biophys Res Commun 2004 320 34-38. 

Sabatier F, Roux V, Anfosso F, Camoin L, Sampol J, Dignat-George F. Interaction of endothelial microparticles with monocytic cells in vitro induces tissue factor-dependent procoagulant activity. Blood 2002 99 3962-3970. 

Gonzalez-Quintero VH, Jimenez JJ, Jy W, Mauro LM, Hortman L, O Sullivan MJ, Ahn Y. Elevated plasma endothelial microparticles in preeclampsia. Am J Obstet Gynecol 2003 189 589-593. 

Combes V, Taylor TE, Juhan-Vague I, Mege JL, Mwenechanya J, Tembo M, Grau GE, Molyneux ME. Circulating endothelial microparticles in Malawian children with severe falciparum malaria complicated with coma. JAMA 2004 291 2542-2544. 

Ogura H, Tanaka H, Koh T, Fujita K, Fujimi S, Nakamori Y, Hosotsubo H, Kuwagata Y, Shimazu T, Sugimoto H. Enhanced production of endothelial microparticles with increased binding to leukocytes in patients with severe systemic inflammatory response syndrome. J Traum 2004 56 823-830. 

Heloire F, Weill B, Weber S, Batteux F. Aggregates of endothelial microparticles and platelets circulate in peripheral blood. Variations during stable coronary disease and acute myocardial infarction. Thromb Res 2003 110 173-180. 

As novel assays become available, the overlap between the clinical and nonclinical biomarkers may further increase. For instance, DIVI and some vasculitides have been associated with changes in levels of circulating endothelial microparticles (EMPs). EMPs are a type of small membrane vesicle (0.1-1 mm in diameter) that is released from cells in response to activation, injury, inflammation, and/or apoptosis and can serve as a means of intercellular communication (Rautou et al, 2011). The use of EMPs for biomarker qualification in drug development may gain broader acceptance with the advent of newer technologies such as... 

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