Endoglucanase gene

Yan, Y., Smant, G., Stokkermans, J., Qin, L., Helder, J., Baum, T., Schots, A. and Davis, E. (1998) Genomic organization of four 5-1,4-endoglucanase genes in plant-parasitic cyst nematodes and its evolutionary implications. Gene 220, 61-70. 

Scheirlinck, T., Mahillon, J., Joos, H., Dhaese, P. and Michiels, F. (1989) Integration and expression of alpha-amylase and endoglucanase genes in the Lactobacillus plantarum chromosome. Appl Environ. Microbiol 55(9), 2130-2137. 

To promote the secretion of foreign proteins, the Tat-dependent signal sequence torA from E. coli, connected to the C. thermocellum endoglucanase gene,... 

Bates, E.E., Gilbert, H.J., Hazlewood, G.P., Huckle, J., Laurie, J.I., and Mann, S.P. (1989) Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum. Appl Environ. Microbiol, 55, 2095 -2097. 

Cho, J.S., Choi, Y.J., and Chung, D.K. (2000) Expression of Clostridium thermocellum endoglucanase gene in Lactobacillus gasseri and Lactobacillus johnsonii and characterization of the genetically modified probiotic lacto-bacilli. Curr. Microbiol, 40, 257-263. 

The endoxylanase gene from Thermoanaerobacter strain B6A was sequenced and structural interpretations were deduced from computer analysis of the amino acid sequence (see Table VIII). The endoglucanase is hydrophilic and has compact secondary structure with 30 6-tums and five potential glycosylation sites. 

The gene, cenB, for a second endoglucanase, CenB, of C. fimi, was also cloned in E. coli (11,15). The polypeptide expressed from cenB in... 

Penttila, M., Lehtovaara, R, Nevalainen, H., Bhikhabhai, R., and Knowles, J. 1986. Homology between cellulase genes of Trichoderma reesei complete nucleotide sequence of the endoglucanase I gene. Gene, 45, 253-263. 

Family 12 contains nine genes, all coding for retaining endoglucanases. They include bacterial and fungal genes. The structure of an endocellulase has been determined and it is a jelly roll made up of P-sheets very similar to the structure of a family 11 xylanase [47]. 

In this study, we report comparisons of the activities and thermal stabilities of purified and recombinant P. funiculosum and T. reesei Cel7A enzymes. All assays were conducted in the presence of the catalytic domain of A. cellulolyticus El endoglucanase (Elcd) as expressed in Escherichia coli and used the dialysis saccharification assay (DSA) format reported by Baker and coworkers . We also report the cloning of the cel7A gene from P. funiculosum in E, coli and exploration of several alternative signal peptide configurations. 

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