Thermoanaerobacter strain

The work to be summarized here is preliminary and will be published elsewhere (manuscripts in preparation). Thermoanaerobacter strain B6A produces a xylanase complex diat is inducible by xylose and catabolite repressed by glucose. Noticeably, when mixed with insoluble xylan, glucose-grown cells do not bind xylan whereas, xylose-grown cells bind very tightly. Canonized ferritin was used to identify xylanosome structures in xylose grown cells which are similar to cellulosomes observed in C. thermocellum, Thermoanaerobacter strain B6A produces thermostable cell-bound endoxylanase, which is also excreted. 

The endoxylanase gene from Thermoanaerobacter strain B6A was sequenced and structural interpretations were deduced from computer analysis of the amino acid sequence (see Table VIII). The endoglucanase is hydrophilic and has compact secondary structure with 30 6-tums and five potential glycosylation sites. 

Table VIL Biochemical Properties of Thermoanaerobacter Strain B6A Endoxylanase Cloned in E. coli...

Table Vm. Structural Interpretations Deduced From Amino Acid Sequence Computer Analysis of the Thermoanaerobacter Strain B6A Endoxylanase Gene...

AmylopuUulanase from Thermoanaerobacter Strain B6A. Saha et al. (14) characterized an extraceUular endo-acting amylopuUulanase from Thermoanaerobacter strain B6A which was also isolated from a geothermal site in the USA. The enzyme was produced at high levels on a variety of carbon sources. It was purified to homo-... 

H., Phelps, T.J., and Zhou, J. (2002) Isolation and characterization of metal-reducing Thermoanaerobacter strains... 

The strain used in this study was Thermoanaerobacter BGILI, which is a lactate dehydrogenase-defieient mutant of the thermophilic anaerobic bacterial strain BGl. The strains Thermoanaerobacter BGl and BGILI have been deposited in the German Collection of Microorganisms and Cell Cultures as patented strains. 

YADH and HLADH are less useful for the asymmetric reductirai of open-chain ketones, but this gap is efficiently covered by a range of alcohol dehydrogenases from mesophiUc bacteria, such as Rhodococcus (ADH-A) and Lactobacillus (LBADH, LKADH), and thermophilic Thermoanaerobacter [802] and Thermo-anaerobium (TBADH) strains (Scheme 2.119) [296, 803-806]. Some of these enzymes are remarkably thermostable (up to 85°C) and can tolerate the presence of organic solvents such as /sopropanol, which serves as hydrogen-donor for NADP-recycling in a coupled-substrate approach [807-809]. 

Korkhin Y, Kalb (Gilboa) AJ, Peretz M, Bogin O, Burstein Y, Erolow F (1998) NADP-dependent bacterial alcohol dehydrogenases crystal structure, cofec-tor-binding and cofactor specificity of the ADHs of Clostridium beijerinckii and Thermoanaerobacter brockii J Molec Biol 278 67—981 Kutzenok A, Aschner M (1952) Degenerative proasses in a strain of Clostridium butylicum. J Bacteriol 64 829-836... 

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