Endogenous 0-glucanases

Endogenous microbial enzymes are sometimes utilized to break down their parent cells, and thus extract valuable intracellular materials. For instance, in the production of yeast extract, cells are allowed to autolyse at about pH 5 and 55 0. Proteases are probably the most important class of enzymes involved in autolysis, although others such as glucanases, lipases and nucleases also have... 

Information about endogenous lytic enzymes affecting the cell walls of basidiomycetous yeasts is less extensive. Villanueva and Gacto (41) reported that / -(1 3)-glucanase activity was lacking in cell extracts of... 

The alkali-extracted gum from oats contained a /3-D-glucan as the major component. Some endogenous D-glucanases survived the alkaline treatment so deactivation of these enzymes increased the yield of the j3-D-glucan with a corre-... 

Kawano, S., Tajitna, K., Kono, H., Erata, T., Munekata, M., Takai, M., 2002. Effects of endogenous endo-(3-l,4-glucanase on cellulose biosynthesis in Acetobacter xylinum ATCC 23769. Journal of Bioscience and Bioengineering 94, 275—281. 

Among other activities, glucanase GV (a commercial enzyme preparation of fungal origin) contains an cndo-P-l,4-D-glucanase, which could be separated from an endogenous cxo-P-l,3-D-glucanase by gel filtration. ... 

A purified e t/o- -D-glucanase from barley was found to be highly specific for the endogenous /S-D-glucan (either in free or dyed form). The enzyme had no action on - 3)- or -(1 4)-linked D-glucans and was inhibited by... 

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