Encore synthesizer

An intermediate level of sophistication was achieved by semiautomated sorting of radiofrequency tagged containers (e.g., MicroKans,8 Lanterns11). A simple instrument that integrates the process of one-dimensional necklace encoding by creating a linear sequences of solid-phase particles is the Encore synthesizer. 

The current version of the Encore synthesizer has been designed for the synthesis of up to 960 compounds using an algorithm to handle 10,8, and 12 building blocks in the first, second, and third combinatorial steps, respectively (Fig. 2). The total number of combinatorial compounds produced this way is n = 10 x 8 x 12 = 960. For the first combinatorial step, 10 reaction vessels are charged with 96 (8 x 12) solid-phase supports and the first set of building blocks is chemically attached to solid-phase supports. After this step, all 96 particles per reaction vessel are identical and particles from different reaction vessels differ only by the kind of first building block attached to solid-phase particles. 

The algorithm described above is for a three-step combinatorial synthesis. However, the method is not limited to only three-step combinatorial libraries the solid-phase support can be derivatized before the directed split-and-pool synthesis on the Encore synthesizer. The necklace coding can also be a very useful tool during the chemistry development process. 

The Encore synthesizer has been designed to integrate the process of handling of solid-phase particles in combinatorial synthesis. The current version of the synthesizer enables synthesis in batches of up to 960 compounds on SynPhase Lanterns. The Encore synthesizer facilitates the sorting of Lanterns into sequences (necklace coding) and the plating of the Lanterns after completion of the synthesis for final release of compounds from Lanterns. The Encore synthesizer consists of the following five tools. 

Combinatorial chemistry has moved from specially centralized laboratories, often equipped with multimillion-dollar robots, onto the bench of individual medicinal chemists. This change in direction requires the availability of personal chemistry tools that are simple to operate, easy to arrange in the laboratory, and reasonably priced. Such instruments are now available for the effective synthesis of combinatorial libraries. The Encore synthesizer represents a simple and efficient personal chemistry tool that allows the execution of directed split-and-pool combinatorial synthesis. The current version of the Encore synthesizer is designed for solid-phase synthesis on SynPhase Lanterns however, it can be modified for synthesis on alternative solid supports such as resin plugs from Polymer Laboratories (e.g., StratoSpheres Plugs). 

Although he claimed that cette synthese n a pas encore ete realisee , it had been achieved by Hennell from ethylene (1826-8 see p. 349). Berthelot later, in reply to Meldola," said HennelFs work had become legendary and that he did not re-form alcohol, whereas Hennell, by distilling ethylsulphuric acid with water, recovered all the alcohol corresponding with the ethylene. Berthelot similarly formed isopropyl alcohol from propylene. 

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