Encoded self-assembling chemical

Figure 4-5 PNA-encoded self-assembling chemical arrays, (a) Each small molecule was labeled with a PNA tag as well as a fluorophore. (b) The tagged molecules were hybridized with their complementary sequences on a decoding array, (c) Incubation of the tagged molecule library with a protein sample generated a protein-small molecule conjugate, which could be separated by using size-exclusion chromatography. Structural information on the bound small molecule was decoded by the decoding array.

Figure 4-6 DNA-encoded self-assembling chemical libraries.

Melkko, S., Scheuermann, J., Dumelin, C. E., Neri, D. (2004). Encoded self-assembling chemical libraries. Nat. Biotechnol, 22, 568-574. 

This concept has been utilized by Neri s group in synthesizing DNA-recorded libraries called encoded self-assembling chemical (ESAC) libraries, and by Hamilton s group in constructing a library of bidentate fragments for binding to streptavidin. As a matter of fact, these libraries are not synthesized, but assembled by combinatorial DNA hybridization. 

DNA-Recorded Synthesis Assisted Libraries Encoded Self-assembly chemical library... 

The types of molecules synthesized by biotechnological techniques are restricted to those biomolecules whose stmctures can be encoded in the DNA of organisms capable of translating them into functional nanomaterials. Other types of molecules and nanomaterials can be synthesized by chemical synthetic approaches, such as covalent syntheses and molecular self-assembly of molecular units. 

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