Encoded self-assembling chemical libraries

Figure 4-6 DNA-encoded self-assembling chemical libraries.

Melkko, S., Scheuermann, J., Dumelin, C. E., Neri, D. (2004). Encoded self-assembling chemical libraries. Nat. Biotechnol, 22, 568-574. 

DNA-Recorded Synthesis Assisted Libraries Encoded Self-assembly chemical library... 

Figure 4-5 PNA-encoded self-assembling chemical arrays, (a) Each small molecule was labeled with a PNA tag as well as a fluorophore. (b) The tagged molecules were hybridized with their complementary sequences on a decoding array, (c) Incubation of the tagged molecule library with a protein sample generated a protein-small molecule conjugate, which could be separated by using size-exclusion chromatography. Structural information on the bound small molecule was decoded by the decoding array.

This concept has been utilized by Neri s group in synthesizing DNA-recorded libraries called encoded self-assembling chemical (ESAC) libraries, and by Hamilton s group in constructing a library of bidentate fragments for binding to streptavidin. As a matter of fact, these libraries are not synthesized, but assembled by combinatorial DNA hybridization. 

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