Encoded combinatorial library binary encoding using

Figure 3. Operators of genetic algorithms as they may be applied to the encoding of molecules from combinatorial libraries. The changes in the bit-strings are indicated by the use of bold-face type. The tripeptoid example from figure 2 has been chosen to illustrate the DNA-like crossover with binary bit strings...

Identification of active library components is done by micro sequencing (peptide libraries) or by incorporation and recognition of the tag associated with each bead (nonpeptide libraries). An example of this latter approach, known as encoded combinatorial synthesis [19], may use the dialkylamine tagging system shown in Fig. 7. The amine group of the tag addition site is acylated with iminodi-acetic anhydride to yield a pentafluorophenyl ester derivative. The active ester is reacted with a binary mixture of secondary alkylamines, leading to a tag polymer. 

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