Enantioselective liquid chromatography studies

Campanero, M.A. Lopez Ocariz, A. Garcia Quetglas, E. Sadaba, B. Azanza, J.R. Determination of ketorolac enantiomers in plasma using enantioselective liquid chromatography. Application to pharmacokinetic studies. Chromatographia 1998, 48 (3 ), 203-208. 

S. Allenmark, A. Ohlsson, Enantioselectivity of Lipase-Catalyzed Hydrolysis of Some 2-Chloroethyl 2-Arylpropanoates Studied by Chiral Reversed-Phase Liquid Chromatography , Chirality 1992, 4, 98-102. 

Despite the long recognition of chirality in POPs, it has only been fairly recently that studies of chiral POPs have been possible, after the introduction of analytical technology for stereoisomer separation by enantioselective gas chromatography (GC), high performance liquid... 

There are many other useful analytical methods. Chromatographic methods such as gas chromatography (GC) and high-performance liquid chromatography (HPLC) are used daily for identification and estimation of the purity of a synthetic product. Chiroptical methods, such as circular dichroism (CD) spectroscopy, are also important especially in studying the relationships between absolute configuration and bioactivity of biofunctional molecules. In later chapters I will give some examples of application of CD spectroscopy in enantioselective synthesis. 

Jabor, V.A.P. Coelho, E.B. Ifa, D.R. Bonato, P.S. dos Santos, N.A.G. Lanchote, V.L. Enantioselective determination of lercanidipine in human plasma for pharmacokinetic studies by normal-phase liquid chromatography-tandem mass spectrometry, J.Chromatogr.B, 2003, 796, 429-437. 

Toribio L, Del Nozal Ml, Bernal JL, Alonso C, Jimenez JJ (2005) Comparative study of the enantioselective separation of several antiulcer drugs by high-performance liquid chromatography and supercritical fluid chromatography. J Chromatogr A 1091 118-123... 

Regarding to the separation of enantiomers, the preparative application of CCC can be of great interest since this technique offers the possibility to produce enan-tiomerically pure compounds at a lower cost compared to conventional liquid chromatography. As for other enantioselective separation techniques, in CCC, a chiral selector (CS) is needed. To produce the enantioselective environment able to separate enantiomers, it is preferably added to the liquid stationary phase. The chiral selector is designed to be confined in the stationary phase thanks to its solubility properties, while the racemate is partitioned between the two phases of the biphasic solvent system. Moreover, the CS must preserve its enantioselectiv-ity in the biphasic liquid chosen. Encountering a combination of solvent system/CS, adapted to the analyte under study, which fulfill the specified requirements is not an easy task. This is the main reason for the few publications released in this field [20, 21]. 

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