Eluant tartaric acid

Eluant Tartaric acid (0.lmol I, fr-MgSO, (0.01 mol )+NaOII (0.lmol )... 

Polyspher IC AN-1 from Merck AG (Darmstadt, Germany), consisting of a hydrophilic polymethacrylate packing with a particle diameter of 12 pm, exhibits a similar selectivity. Depending on the type of application, this column can be operated with eluants based on phthalic acid, p-hydroxybenzoic acid, tartaric acid, oxalic acid, or boric acid. Figure 3-16 shows a typical chromatogram obtained with a phthalic acid eluant. Under these chromatographic conditions (pH 4) orthophosphate elutes as a monovalent anion ahead of chloride. 

The chromatogram shown in Fig. 3-53 exemplifies how strongly the selectivity of a separation system depends on the eluant. A silica ion exchanger, TSK GEL IC-SW from Toyo Soda Company, was employed as a stationary phase. The separation of chlorate and nitrate that is achieved using 1 mmol/L tartaric acid eluant is remarkable. Such a separation is not possible on a polymer-based latex-... 

Fig. 3-53. Separation of various inorganic anions on a TSK Gel IC-SW silica-based anion exchanger. — Eluant 1 mmol/L tartaric acid (pH 3.2) flow rate ...

Fig. 3 -125. Comparison between the retention behavior of inorganic anions and several carboxylic acids. -Separator columns 2 lonPac AS4 eluant 2.8 mmol/L NaHCOs + 2.2 mmol/L Na2COj flow rate 1.6 mL/min detection suppressed conductivity injection 50 pL solute concentrations A) 1.5 mg/L fluoride (1), 2 mg/L chloride (2), 5 mg/L each of orthophosphate (3) and bromide (4), 10 mg/L nitrate (5), and 12.5 mg/L sulfate (6), B) 5 mg/L formic acid (7), 40 mg/L benzoic acid (8), 20 mg/L succinic acid (9), 10 mg/L malonic acid (10), 20 mg/L each of maleic acid (11), tartaric acid (12), and oxalic acid (13).

Polymethacrylate and polyvinyl resins play only a secondary role in the manufacturing of cation exchangers. Currently, the only polymethacrylate-based cation exchanger on the market is offered by Sykam (Gauting, Germany) under the trade name LCA K02. This column differs from its PS/DVB-analogue (see Table 4-1) only in particle size (5-pm) and ion-exchange capacity (0.4 mequiv/g). Used with a tartaric acid eluant, this phase is preferred for the analysis of transition metals. 

Fig. 4-30. Simultaneous separation of alkali metals, alkaline-earth metals, and ammonium on PBDMA-coated silica with a tartaric acid/pyridine-2,6-dicarboxylic acid eluant. — Separator column Metrosep Cation 1-2 eluant 5 mmol/L tartaric acid -t 0.75 mmol/L pyridine-2,6-dicarboxylic acid flow rate 1 mL/min detection direct conductivity injection volume 10 pL solute concentrations ...

Fig. 4-32. Separation of transition metals together with alkali- and alkaline-earth metals on PBDMA-coated silica. - Separator column LiChrosil IC CA column temperature 40°C eluant 2 mmol/L tartaric acid a- 0.5 mmol/L oxalic acid flow rate 1 mL/min detection ...

The LiChrosil IC CA column has been successfully employed in the analysis of ethanolamines, which can be eluted with high efficiency in less than seven minutes with a tartaric acid/dipicolinic acid eluant. The chromatogram in Fig. 4-33 shows the three ethanolamines eluting with high chromatographic efficiencies within seven minutes. 

Fig. 4-45. Separation of alkaline-earth metals on Shimpack IC-Cl. -Eluant 4 mmol/L tartaric acid -f 2 mmol/L ethylenediamine flow rate ...

Fig. 4 -49. Separation of transition metals on a polymethacrylate-based cation exchanger. - Separator column Sykam LCA K02 eluant 0.1 mol/L tartaric acid, pH 2.95 with NaOH flow rate 2 mL/min detection photomet at 500 nm after reaction with PAR and ZnEDTA injection volume 100 pL solute concentrations ...

Fig. 5-3. Separation of carboxylic acids and hydroxycarboxylic acids on lonPac ICE-AS6. - Eluant 0.4 mmol/L heptafluorobutyric acid flow rate 0.4 mL/min detection suppressed conductivity injection volume 50 pL solute concentrations 5 mg/L oxalic acid (1), 10 mg/L tartaric acid (2),...

Apart from the obligatory membrane filtration (0.45 pm), the only sample preparation in the analysis of rain water, ground water, and swimming pool water is a dilution with de-ionized water. Figure 9-9 shows a cation chromatogram of a rain water sample [42] that applies non-suppressed conductivity detection the sample was injected without any dilution because of its low electrolyte content. Metrosep Cation 1-2 served as the separator column and the eluant was a mixture of tartaric acid and pyridine-2,6-dicarboxylic acid. 

Separator column RT 300-6.5 Polyspher CHCA column temperature 85 °C eluant deionized water flow rate 0.6 mL/ min detection Rl injection volume 20 pL sample dry white wine with a total sugar content of 0.1 g/L, spiked with tartaric acid, lactic acid, and malic acid to yield 17.6 g/L total acid peaks ... 

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