Elongase gene

Table 4.5 Number of transcripts reported for some elongase genes probably involved in pheromone biosynthesis. The cytological localization (cyt) of the genes is reported. Relative male vs. female expression is indicated (Parisi et al., 2004 Mackay et al., 2005).

Major advances in the cloning and manipulation of fatty acid desaturase and elongase genes from various organisms have been made during the past several years, and these recombinant enzymes offer the prospect of producing a desired... 

Chen, R., Matsui, K., Ogawa, M., Oe, M., Ochiai, M., Kawashima, H., Sakuradani, E., Shimizu, S., Ishimoto, M., Hayashi, M., et al., 2006. Expression of A6, A5 desaturase and GLELO elongase genes from Mortierella alpina for production of arachidonic acid in soybean [Glycine max (L.) Merrill] seeds. Plant Sci. 170, 399-406. 

Lastly, a C20-elongase gene and a A5-fatty acid elongase were identified and isolated from Pavlova viridis and Pavlova salina, respectively (Niu et al., 2009 Robert et al., 2009). 

Niu, Y., Kong, J., Fu, L., Yang, J., and Xu, Y. (2009) Identification of a novel C20-elongase gene from the marine microalgae Pavlova viridis and its expression in Escherichia coli. Mar. Biotechnol., 11, 17-23. 

It has been assumed for many years that the first step in the elongase cycle, the condensation step, regulates the chain length of the final product. Direct evidence for this has been obtained in Arabidopsis, where the fatty acid elongationl (FAE1) gene encodes for the condensing enzyme (Millar and Kunst, 1997). The... 

An extensive study of elongases will take time, but is necessary to get a better insight into their roles. In this respect, the use of RNAi knockdown of genes specifically induced in biosynthesis tissues (oenocytes) could allow relatively fast determination of - at least -the genes involved. 

Chertemps, T., Duportets, L., Labeur, C., Ueyama, M. and Wicker-Thomas, C. (2006). A female-specific desaturase gene responsible for diene hydrocarbon biosynthesis and courtship behaviour in Drosophila melanogaster. Insect Mol. Biol., 15,465—473. Chertemps, T., Duportets, L., Labeur, C. and Wicker-Thomas, C. (2005). A new elongase selectively expressed in Drosophila male reproductive system. Biochem. Biophys. Res. Commun., 333,1066-1072. 

Beaudoin, F., Gable, K., Sayanova, O., Dunn, T., Napier, J. A. A Saccharomyces cerevisiae gene required for heterologous fatty add elongase activity encodes a microsomal beta-keto-reductase. J. Biol. Chem. 2002, 277 11481-11488. 

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