Electrophoresis infants

Tissot, J.D., Hohlfeld, P., Forestier, F., Tolsa, J.F., Calame, A., Plouvier, E., Bossart, H., and Schneider, P., 1993b, Plasma/serum protein patterns in human fetuses and infants a study by high-resolution two-dimensional polyacrylamide gel electrophoresis. Appl. Theor. Electroph. 3 183-190. 

Veledo MT, de Frutos M, Diez-Masa JC. Analysis of trace amounts of bovine beta-lactoglobulin in infant formulas by capillary electrophoresis with on-capUlary derivatization and laser-induced fluorescence detection. J Sep Sci 2005 28 941-7. 

Klampfl C. W., and Ahrer, W., Determination of free amino acids in infant food by capillary zone electrophoresis with mass spectrometric detection, Electrophoresis, 22, 1579, 2001. 

Veledo, M. T., Frutos, M., and Diez-Masa, J. C., Analysis of trace amonnts of bovine fS-lactoglobulin in infant formulas by capillary electrophoresis with on-capiUary derivatization and laser-indnced fluorescence detection, /. Sep., Sci., 28, 941, 2005. 

Capillary electrophoresis-ESI-MS (CE-ESI-MS) was proposed for identification and simultaneous quantification of several 5 -monophosphate ribonucleotides in infant formula samples [26], The BGE was a 30 mM ammonium formate-ammonia medium (pH 9.6). Electrophoretic separation was achieved with a voltage of 30 kV (positive CE mode), with an initial ramp of 7 s. The temperature of the capillary was kept constant at 25 C. The mass spectrometer was operated in the negative-ion mode. The optimized fragmenter voltage was 100 V for all analytes. For quantification, SIM mode was selected. The miz values corresponding to the [M-H] ions were 346 for AMP, 322 for CMP, 362 for GMP, 323 for UMP, and 347 for IMP. The results indicated that CE coupled to MS offered an alternative to other chromatographic methods for analysis of nucleotides and related compounds in infant formula samples. 

Gonzalo, E. R., J. Dommguez-Alvarez, M. Mateos-Vivas, D. Garcia-Gomez, and R. Carabias-Martmez. 2014. A validated method for the determination of nucleotides in infant formulas by capillary electrophoresis coupled to mass spectrometry. Electrophoresis 35 1677-1684. 

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