Electronic absorption spectroscopy probes

Probing Metalloproteins Electronic absorption spectroscopy of copper proteins, 226, 1 electronic absorption spectroscopy of nonheme iron proteins, 226, 33 cobalt as probe and label of proteins, 226, 52 biochemical and spectroscopic probes of mercury(ii) coordination environments in proteins, 226, 71 low-temperature optical spectroscopy metalloprotein structure and dynamics, 226, 97 nanosecond transient absorption spectroscopy, 226, 119 nanosecond time-resolved absorption and polarization dichroism spectroscopies, 226, 147 real-time spectroscopic techniques for probing conformational dynamics of heme proteins, 226, 177 variable-temperature magnetic circular dichroism, 226, 199 linear dichroism, 226, 232 infrared spectroscopy, 226, 259 Fourier transform infrared spectroscopy, 226, 289 infrared circular dichroism, 226, 306 Raman and resonance Raman spectroscopy, 226, 319 protein structure from ultraviolet resonance Raman spectroscopy, 226, 374 single-crystal micro-Raman spectroscopy, 226, 397 nanosecond time-resolved resonance Raman spectroscopy, 226, 409 techniques for obtaining resonance Raman spectra of metalloproteins, 226, 431 Raman optical activity, 226, 470 surface-enhanced resonance Raman scattering, 226, 482 luminescence... 

Electronic absorption spectroscopy charge transfer transitions, 19 71 d-d transitions, 19 70, 71 flavocytochrome b, 36 269-271 intraligand transitions, 19 71-80 of organometallics, 19 69-80 Electronic coupling, between donor and acceptor wave functions, 41 278 Electronic nuclear double resonance spectroscopy, molybdenum center probes, 40 13... 

Zsila, F., Bikadi, Z., and Simonyi, M., Probing the binding of the flavonoid, quercetin to human serum albumin by circular dichroism, electronic absorption spectroscopy and molecular modelling methods, Biochem. Pharmacol, 65, 447, 2003. 

One of the most commonly apphed types of spectroscopy in the picosecond realm is pump-probe electronic absorption spectroscopy. The absorption spectra of reactive intermediates are usually just as featureless as those of the other two time domains described in this volume. It is simply the inherent nature of these spectra in condensed phases, most typically in solution. Spectroscopic studies in solution most closely mimic reaction conditions that reactive intermediates may find themselves involved in when they are formed and consumed during the course of an organic chemical reaction. 

D. J. Tannor Prof. Rabitz, from the time-domain formulation of conventional electronic absorption spectroscopy, we know that the information content in the wavepacket autocorrelation function is identical to that in the high-resolution spectrum. Yet it is clear that the wavepacket autocorrelation function only directly probes the times at... 

The electronic structure of transition metal clusters is difficult to probe by electronic absorption spectroscopy, which has proved so fhiitful in revealing the d orbital orderings of classical complexes, because of the multiplicity of overlapping absorption bands. The one-to-one correlation between occupied orbitals and PE bands means that PES can give valuable information on the smaller clusters. The information obtained has given support to the semiempirical theoretical treatments of this class of molecules. 

Sample purity is a key concern. The NRVS experiment is a bulk technique sampling all Fe nuclei, and impurities that also contain the probe nucleus may confound quantitative data interpretation. Impurities may be introduced during sample preparation or may result from sample instability during measurement. Because of this, care must be taken to ensure purity and reproducibility as judged by Mossbauer (see Mdssbauer Spectroscopy), single-crystal X-ray diffraction, electronic absorption spectroscopy (see Electronic Spectroscopy), Raman spectroscopy or other qnalitative techniqnes. 

Electronic absorption spectroscopy (ABS) Energies, intensities, and band shapes Direct probe of ligand field and charge transfer excited states... 

Electronic coupling can be probed using electrochemistry. Brewer and Richter investigated bimetallic complexes of the form [(bpy)20s(BL)0s(bpy)2] " (BE = 2,3-bis(2-pyridyl)pyrazine (dpp), 2,3-bis(2-pyridyl)quinoxaline (dpq) or 2,3-bis(2-pyridyl)benzoquinoxaline (dpb), and bpy = 2,2 -bipyridine) by electronic absorption spectroscopy and spectroelectrochemistry. When BE = dpb, the 1/2... 

Ultraviolet photoelectron spectroscopy (UPS) involves ionization of valence electrons which probes metal-ligand bonding and change with ionization (from resonance effects as photon energy is scanned through metal M edge), thus directly studying redox processes in metal complexes allows for study of closed shell systems which are inaccessible via electronic absorption spectroscopy ... 

The term solvatochromism was introduced in 1922 by Hantzshlater [45] to explain the influence of the solvent on electronic absorption spectra. In 1951 Brooker et al. [46] suggested that the solvatochromic effect induced by a solvent on certain probes that have a strong absorption in the UV/Vis could be used as a visual indicator of the solvent polarity. One of the main reasons that spectroscopic measurements are such a popular method to obtain empirical polarity parameters is the simplicity of the experiment Electronic absorption spectroscopy is a readily available tool that requires little speciahst skill to operate. Hence, several of these scales have been used with ILs. 

The most sensitive probe of tc(CsW-CsC) conjugation in the metallabutadiynes is electronic-absorption spectroscopy. The lowest-energy absorption band of W(CH)L4X complexes has been assigned as [d , k (W=C)], the terminating orbital of which is of the... 

Pump-probe absorption experiments on the femtosecond time scale generally fall into two effective types, depending on the duration and spectral width of the pump pulse. If tlie pump spectrum is significantly narrower in width than the electronic absorption line shape, transient hole-burning spectroscopy [101. 102. 103. 104. 105. 106. 107. 108. 109. 110. 111. 112 and 113] can be perfomied. The second type of experiment, dynamic absorption spectroscopy [57, 114. 115. 116. 117. 118. 119. 120. 121 and 122], can be perfomied if the pump and probe pulses are short compared to tlie period of the vibrational modes that are coupled to the electronic transition. 

Vidal F, Busson B, Tadjeddine A, Peremans A. 2003. Effect of a static electric field on the vibrational and electronic properties of a compressed CO adlayer on Pt(l 10) in nonaqueous electrolyte as probed by infia ed reflection-absorption spectroscopy and infi ared-visible sum-fi equency generation spectroscopy. J Chem Phys 119 12492-12498. 

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