Electromigration methods

For obtaining internal or external mobilities, the corresponding transport numbers are usually measured. There are several methods for determining transport numbers in molten salts that is, the Kleimn method (countercurrent electromigration method or column method), the Hittorf method (disk method), the zone electromigration method (layer method), the emf method, and the moving boundary method. These are described in a comprehensive review. ... 

There is difficulty in defining the absolute mobilities of the constituent ions in a molten salt, since it does not contain fixed particles that could serve as a coordinate reference. Experimental means for measuring external transport numbers or external mobilities are scarce, although the zone electromigration method (layer method) and the improved Hittorf method may be used. In addition, external mobilities in molten salts cannot be easily calculated, even from molecular dynamics simulation. 

Novotny, M., Soini, M., and Stefansson, M., Chiral separation through capillary electromigration methods, Anal. Chem., 66, 646A, 1994. 

Owing to its potential of performing extremely high-efficiency separations, robustness of the equipment, automation, ease of use and flexibility, electromigration methods, i.e. CE have widely been applied to different problems in analytical chemistry. It is considered to be a complementary or even an alternative technique to established chromatographic techniques such as HPLC, GC and others. 

Electromigration methods origins, principles, and applications TABLE 17.2 (continued)... 

The contribution of electromigration methods to analytical chemistry and biopharmaceutical science has been very significant over the... 

Electromigration methods compose a family of analytical separation methods based on differences in the mobilities of charged analytes in the electric field. In this chapter, we discuss mainly such electromigration methods that are performed in thin capillaries with inner diameter (i.d.) <0.1 mm. These methods are commonly known as capillary electrophoretic methods where the most important modes are capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), capillary gel electrophoresis (CGE), and capillary electrochromatography (CEC). 

The best known CE buffer ingredient is sodium dodecyl sulfate (SDS) proposed by Terabe [10,11]. SDS forms micelles and the separation of neutral analytes is achieved by their partitioning between the buffer and the SDS micelles, that is, by their hydrophobicity. This is the basis of MEKC and the mobility of analytes correlates linearly well with logP values, where P is the octanol/water partition ratio. Many other buffer ingredients have been proposed. Most of them implement hydrophobic interactions between the analytes and the buffer ingredients but also ciral selectors have been used as well as various affinity probes. Interest in the ILs used as buffer additives in capillary electromigration methods is due to the fact that they could provide an alternative separation mechanism to two currently implemented mechanisms in CE which are based either on the charge to mass ratio or on the hydrophobicity of the analytes. 

Volume 60 Advanced Chromatographic and Electromigration Methods in BioSciences edited by Z. Deyl, I. Miksik, F. Tagliaro and E. Tesarova... 

J. F. Banks, Protein analysis, in Advanced Chromatographic and Electromigration Methods in BioSciences (Z. Deyl, I. Miksik, F. Tagliaro, E. Tesarova, eds.). Journal of Chromaography Library Vol. 60, Elsevier, Amsterdam, 1998, pp. 525-573. 

Deyl D, Miksik I, Tesarova E. In Deyl Z. Miksik I. Tagliano F. Tesarova E. eds. Advanced Chromatographic and Electromigration Methods in Biosciences Amsterdam Elsevier, 1998 166-9. 

What is an advantage of the electromigrative method of adding sample ... 

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