EGFP Expression

Peptide-induced membrane pcrmcabilization. (A) Stable EGFP expressing HeLa cells 315... 

Fig. 9 PCI-induced transfection utilizing branched and linear PEI as vector. HT116 colon carcinoma cells were treated with AlPcS (20 (tg/mL) for 18 h followed by a 4-h incubation with an plasmid encoding EGFP and complexed with 25 kDa branched or 22 kDa linear PEI at an N P ratio of 4 before light exposure. EGFP expression was measure by flow cytometry 2 days later. The photochemical treatment caused 40-60% cytotoxicity...

Fig. 6. Effect of a 13.5-h cis- or trans -DDP treatment on doxycycline-inducible EGFP expression in HeLa Tet-on cells. The fluorescence values were divided by total protein to correct for variations in cell count.

Fig. 7. IC50 vs. LC50 values for HeLa cells treated with a series of platinum complexes. LC50 was determined by a colony-counting assay. /C50 was the platinum concentration at which EGFP expression was reduced to 50% of control.

The a1B-AR in the mouse brain was expressed predominantly in the neuronal cells of the cerebral cortex (Fig. 2). We confirmed neuronal expression by using an antibody directed against neurons (NEUN) and showed co-localization with the EGFP expression. This is consistent with the in situ hybridization data, which predicted that the cerebral cortex was one area of the rat brain that contained a high population of this oq-AR subtype (Table 1). Expression was evenly distributed through the different laminae of the cortex. For the a1A-AR, expression was also evident in the neuronal cell populations of the cerebral cortex, but a different neuronal cell type was even more pronounced in expression (Fig. 3). Differences in expression between the a1A- and a1B-ARs is not caused by differences in... 

Detection of EGFP Expressing Antigen Presenting Ceiis After Intradermai Vaccination with PEGFPL-33A DNA Liposomes... 

Determination of the Percentage of eGFP-expressing Cells using Flow Cytometry... 

To quantify eGFP expression in cell lysates prepared as described in Subheading 3.10. Step 1 for adherent or in Subheading 3.10 step 2 for suspension cells, transfer 50pl cell... 

Cell seeding in a clear bottom black-walled plate enables eGFP expression measurements in living cells. This could be used to study the kinetics of eGFP expression post-transfection. 

Antisense Experiment" Observed Toxicity Approximate Binding Constant % Decrease in EGFP Expression... 

Howard and Kjems (2006) reported that chitosan/siRNA complexes effectively silenced EGFP expression in both H1299 human lung carcinoma cells (77.9% reduction) and murine peritoneal macrophages (89.3% reduction). Katas and Alpar (2006) reported that preparation methods such as... 

Incubating cells with fluorophore- Mirkin et al. [64] labeled DNA conjugated to nanoparticles resulted in efficient uptake of the particles. Protein expression was controlled by conjugation of antisense DNA with GNPs. EGFP-expressing C166 cells incubated with antisense DNA-functionalized nanoparticles (ASNP) resulted in reduction of fluorescence intensity in cells incubated with ASNP... 

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