Effect of VSV on Cellular and Viral DNA Synthesis

Effect of VSV on Cellular and Viral DNA Synthesis 5.7.1. Parameters of Cellular DNA Synthesis Inhibition 

In their studies on the effect of VSV (Indiana serotype) on interferon synthesis, Wagner and Huang (1966) noted that the incorporation of [ HJthymidine into acid-precipitable material was drastically inhibited in VSV-infected compared to uninfected Krebs-2 ascites tumor cells. Yaoi and Amano (1970) infected chicken embryo 

Viral Functions Required to Inhibit Cellular DNA Synthesis 

The effect of VSV on cellular DNA synthesis could result from perturbation of cellular structures similar to those conceivably involved in inhibition of RNA and protein synthesis. The effect is unlikely to be at the limiting cell membrane because McGowan and Wagner (1981) found that VSV infection does not significantly alter uptake of thymidine into soluble intracellular pools of nucleosides. 

Since the effect of VSV on initiation of DNA synthesis cannot be measured directly, McGowan and Wagner (1981) were prompted to examine more closely the ability of VSV to inhibit DNA chain elongation in VSV-infected MPC-11 cells. Analyses in alkaline sucrose gradients of radioactive pulse-chased DNA from VSV-infected and uninfected cells indicated that, although [ HJthymidine incorporation into DNA was inhibited by VSV infection, the process of DNA chain elongation can continue in infected cells. 

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