E-rosettes

Figure 1335. Packed column and internals, (a) Example packed column with a variety of internals [Chen, Chem. Eng. 40, (5 Mar. 1984)]. (b) Packing support and redistributor assembly, (c) Trough-type liquid distributor, (d) Perforated pipe distributor, (e) Rosette redistributor for small towers. (0 Hold-down plate, particularly for low density packing.

CD2, the E rosette receptor, is an extremely broad T-cell marker. Antibodies to it immunolabel the vast majority of T and natural killer cell malignancies but may require signal amplification by the Immunomax method for paraffin section use. Some thymic B cells are also CD2 positive. 

Probably the earliest cellular change that can be detected by 1 week following thymectomy in adult mice is the disappearance of splenic lymphocytes that form azathioprine-sensitive E rosettes (Bach and Dardenne, 1973). This observation enabled Bach and Dardenne to subsequently develop an in vitro bioassay that was utilized for the detection of circulating levels of thymic hormonelike bioactivity (see Section 7.1.1). Thus, there is now ample evidence that in the adult the thymus continues to manifest an important long-term role with regard to the maintenance and regulation of the immune system. 

Madsen, M., and Johnsen, H.E., 1979, A methodological study of E-rosette formation using AET-treated sheep red blood cells, J. Immunol, Methods, 27 61. 

Preparation of cell extracts. MOLT 4F is a human T-cell line established from a patient with acute lymphocytic leukemia (13) and has since been maintained in our laboratory. Characteristically, MOLT 4F cells form rosettes with sheep red blood cells (E-rosettes). They do not synthesize immunoglobulin and grow as a single cell suspension. RPMI 1788 is a human B-cell line established from the peripheral blood of a healthy volunteer. These cells produce immunoglobulin, have complement receptors and grow as clusters in suspension culture. Characteristics of these cell lines have recently been summarized by Moore (14). These cells were maintained in medium RPMI 1640 with 10% fetal calf serum and penicillin and streptomycin. The viability of the cells measured by a trypan blue... 

Galili, U., and Schlesinger, M., 1974, The formation of stable E rosettes after neuraminidase treatment of either human peripheral blood lymphocytes or of sheep red blood cells, 7. Immunol. 112 1628. 

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