Dynamic laser light scattering

Selected entries from Methods in Enzymology [vol, page(s)] Anisotropy effects, 261, 427-430 determination by dynamic laser light scattering (quasi-elastic light scattering), 261, 432-433 determination for nucleic acids by NMR [accuracy, 261, 432-433 algorithms, 261, 11-13, 425, 430 carbon-13 relaxation, 261, 11-12, 422-426, 431, 434-435 cross-relaxation rates, 261,419-422, 435 error sources, 261, 430-432 phosphorus-31 relaxation, 261, 426-427, 431 proton relaxation, 261,51,418-422 relaxation matrix calculations, 261,12] deuterium solvent viscosity effects, 261,433 effect... 

P Schurtenberger, NA Mazer, W Kanzig. Dynamic laser-light-scattering studies of the micelle to vesicle transition in a model and native bile. Hepatology 4 143-147, 1984. 

Kadima, W.L. Ogendal, R., Bauer, N., Kaarsholm, K., Brodersen, Hansen, J.F., Porting, P. (1993). The Influence of ionic strength and ph on the aggregation properties of zinc-free insulin studied by static and dynamic laser light scattering. Biopolymers, 33, 1643-1657. 

Combination of static and dynamic laser light scattering is also useful to determine not only the size distribution but also the particle structure of polymer colloids such as the adsorbed surfactant layer thickness [73] and the formation of nanoparticles [74,75]. A recently developed method of determining the density of polymer particles is outlined below to illustrate the usefulness of laser light scattering as a powerful analytical tool for investigating more sophisticated colloidal problems [76-78]. 

A Comparison of Methods for Determining Macromolecular Folydispersity from Dynamic Laser Light Scattering Data... 

II. Light scattering Dynamic Laser-light scattering 107... 

Note that it is more common to observe multiple molecular condensates in conventional techniques such as total intensity and dynamic laser light scattering, which are not well suited to such a dilute solution. The term condensation was intended to make the distinction with the usual aggregation or precipitation, which indicate the situation in which the aggregate is of finite size and orderly morphology [6,7]. 

Particle diameter Inductively coupled plasma-optical emission spectrometry (ICP-OES) Fourier transform infrared spectrometry Mass spectrometry X-ray fluorescence Extended X-ray absorption fine structure (EXAFS) spectroscopy X-ray absorption near edge (XANES) spectroscopy Static and dynamic laser light scattering Scanning probe technologies... 

Size distribution Static and dynamic laser light scattering Photon correlation spectroscopy... 

Three of the experiments are completely new, and all make use of optical measurements. One involves a temperature study of the birefringence in a liquid crystal to determine the evolution of nematic order as one approaches the transition to an isotropic phase. The second uses dynamic laser light scattering from an aqueous dispersion of polystyrene spheres to determine the autocorrelation function that characterizes the size of these particles. The third is a study of the absorption and fluorescence spectra of CdSe nanocrystals (quantum dots) and involves modeling of these in terms of simple quantum mechanical concepts. 

Mantovani F, Grassi M, Colombo I, Lapasin R. A combination of vapor sorption and dynamic laser light scattering methods for the determination of the Flory parameter chi and the crosslink density of a powdered polymeric gel. Fluid Phase Equilib 2000 167(1) 63— 81. 

Finally, the liposomes are sterilized by filtration (0.45- or 0.2-p,m sterile filters). Mean hydrodynamic diameters of vesicles (liposomes, nanospheres, nanobeads) can be determined with dynamic laser light scattering instruments, e.g. the NICOMP... 

Siddiqu and Wu [31] used a dynamic laser light scattering method to characterise an unfractionated PI in chloroform at 25 °C. The relatively small angular and concentration dependencies and translational diffusion coefficients measured by this technique enabled the PI to be characterised from only one measurement at a finite concentration and small scattering angle. Thus, this method could be used to characterise the molecular weight distribution of PI from the measured line-width distribution. 

Fig. 9. Two commonly used configurations of the detection optics for both static and dynamic laser light scattering, where Anc is the incident light and f the focal length.

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