Dual expression plasmid

PLASMID DNA-BASED ANTI-TUMOR VACCINES. One of the first reports of an antitumor pDNA-vaccine phase I trial used a dual expression plasmid coding HBS and carcino embryonic antigen (CEA), a protein overexpressed on many epithelial cancers. The plasmid was repetitively injected intramuscularly. Per injection, up to 2mg was applied. Some patients developed a good immune response toward HBS, but there was only a very limited response toward CEA. No objective clinical... 

For other production hosts (yeast, insect, and mammalian cells), standard promoter formats have been used in combination with FITP cloning methods to produce vectors for expression screening (see Section 2.3.2). A particularly interesting development is the use of multipromoter plasmids for expression in two or more hosts from a single vector. The construction of a dual E.coli (T7 promoter) and baculovirus transfer vector (polH promoter) for expression in insect cells has been described (Chambers et al., 2004). A three-promoter vector (T7, plO, and hCMV or CAG promoter) is available from Novagen (pTrlEX ) and its use reported for comparing protein expression in E. coli and insect cells (Xu and Jones, 2004). 

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