DTNB

The rates are essentially independent of the distribution of metal in the MT with similar rates between Zn7—MTm (Cd, Zn)7-MT, and Cd7—MT. The values of the rate constants are ks = 6.9( 0.9) x 10 " s and kf=2.7( 1.2) x 10 s for the holo-protein. The slow rate constant is similar in magnitude to the first-order protein-dependent steps observed for reactions of DTNB (5,5 -dithiobis(2-nitrobenzoate)), EDTA (ethylenediamine tetraacetate), cisplatin, and other reagents, which has been attributed to a rearrangement of the protein. The fast step is more rapid by an order of magnitude, which suggests that other mechanisms are prevailing. 

Savas, M.M., Petering, D.H. and Shaw, C.F. Ill (1991) On the rapid, monophasic reaction of the rabbit liver metaDothionein a-domain with 5,5 -dithiobis (2-nitrobenzoic acid) (DTNB). Inorganic Chemistry, 30, 581-583. 

ND = Not determined DTNB 2,2 -Dithiobis(5-nitropyridine) DTNP 2,2 -Dithiobis(5-nitrobenzoate) DEDC Diethyldithiocarbamate IA Iodoacetate NEM N-ethylmaleimide PCMB p-chloromercuribenzoate 1, 10-PT 1, 10-phenanthroline 8-Q 8-Quinolinol 2, 2 -BP 2, 2 -Bipyridyl. 

Reagents 20 mM Na-K-phosphate buffer pH 7.4, Ellman reagent 5,5"-dithio-bis(p-nitrobenzoic acid) shortly DTNB, or its red analogue 2,2-dithio-... 

Fig. 3.8.6 Spectrophotometric assays of complexes V, I III, II—III, and citrate synthase activity. DTNB Dithio-nitrobenzoic acid, LDH lactic dehydrogenase, ME medium E, MF medium F, MG medium G, MH medium H, PEP phosphoenol pyruvate, PK Pyruvate kinase, TNB thio-nitrobenzoic acid...

Dithiobis-(2-nitorobenzoic) acid (DTNB) as an LCAT inhibitor. 

Ellman s reagent 5,5 -dithiobis(2-nitrobenzoic acid DTNB) reacts quantitatively with - SH groups (Eq. 3-41) to form mixed disulfides with release of a thiolate anion that absorbs light at 412 nm with a molar extinction... 

Measure the thiol content of the pool by mixing 100 pL of the solution with 100 pL of a 0.00 IM solution of DTNB in 0.1M phosphate buffer, pH 7 0 (reaction performed in wells of a microtiter plate)... 

Ellman s reagent, 5,5 -dithio-bis-(2-nitrobenzoic acid), or DTNB, is a compound useful for the quantitative determination of sulfhydryls in solution (Ellman, 1958,1959). The disulfide of Ellman s reagent readily undergoes disulfide exchange with a free sulfhydryl to form a mixed disulfide and release of one molecule of the chromogenic substance 5-sulfido-2-nitrobenzoate, also called 5-thio-2-nitrobenzoic acid (TNB). The intense yellow color produced by the TNB anion can be measured by its absor-... 

A remarkable experiment205) has been performed on the function of myosin light chains in calcium regulation. It shows that rabbit DTNB (5,5-dithiobis-2-nitro-... 

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